FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001323733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001323733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG90.4980630879911455RNA PCR Primer, Index 23 (96% over 30bp)
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA70.387382401770891No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG70.387382401770891No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT70.387382401770891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.387382401770891No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA60.3320420586607637RNA PCR Primer, Index 27 (96% over 30bp)
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC60.3320420586607637No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT60.3320420586607637No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA60.3320420586607637No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT50.2767017155506364No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG50.2767017155506364No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG50.2767017155506364RNA PCR Primer, Index 46 (96% over 28bp)
GTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGG50.2767017155506364No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG50.2767017155506364No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG50.2767017155506364RNA PCR Primer, Index 27 (96% over 30bp)
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA50.2767017155506364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2767017155506364No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA50.2767017155506364No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG40.22136137244050913No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG40.22136137244050913No Hit
GCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAG40.22136137244050913No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG40.22136137244050913RNA PCR Primer, Index 33 (96% over 29bp)
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA40.22136137244050913No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG40.22136137244050913No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT40.22136137244050913No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG40.22136137244050913TruSeq Adapter, Index 3 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.22136137244050913No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT30.16602102933038185No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT30.16602102933038185No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA30.16602102933038185No Hit
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT30.16602102933038185No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG30.16602102933038185No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA30.16602102933038185No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT30.16602102933038185No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG30.16602102933038185No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC30.16602102933038185No Hit
CCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTC30.16602102933038185No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT30.16602102933038185No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC30.16602102933038185No Hit
ATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGT30.16602102933038185No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16602102933038185Clontech SMART CDS Primer II A (100% over 21bp)
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG30.16602102933038185No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA30.16602102933038185RNA PCR Primer, Index 46 (96% over 28bp)
CATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG30.16602102933038185No Hit
TACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAG30.16602102933038185No Hit
CCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTG30.16602102933038185No Hit
ATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGA30.16602102933038185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.16602102933038185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16602102933038185No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA30.16602102933038185No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC30.16602102933038185No Hit
ATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTA20.11068068622025456No Hit
GACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGT20.11068068622025456No Hit
ATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGG20.11068068622025456No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG20.11068068622025456No Hit
TTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAA20.11068068622025456No Hit
GGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAA20.11068068622025456No Hit
GGTAAGAAGTGGGCTAGGGCATTTTTAATCTTAGAGCGAAAGCCTATAAT20.11068068622025456No Hit
TTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTT20.11068068622025456No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT20.11068068622025456No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC20.11068068622025456No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC20.11068068622025456No Hit
GGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGC20.11068068622025456No Hit
CTTTAAATGAGCTAGGGACAAATTAAGGATTCAGAGAACTATTAACACAT20.11068068622025456No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT20.11068068622025456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC20.11068068622025456No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC20.11068068622025456No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG20.11068068622025456No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA20.11068068622025456No Hit
TGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG20.11068068622025456No Hit
GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTT20.11068068622025456No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA20.11068068622025456No Hit
TGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTT20.11068068622025456No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG20.11068068622025456No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA20.11068068622025456No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT20.11068068622025456No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC20.11068068622025456No Hit
GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATAC20.11068068622025456No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA20.11068068622025456RNA PCR Primer, Index 23 (96% over 30bp)
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA20.11068068622025456No Hit
TTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCC20.11068068622025456No Hit
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA20.11068068622025456No Hit
CCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAAC20.11068068622025456No Hit
CTGTACACCTTTTCACTTAATAGAGCGTCTCTTACTTCTCTCAGTCATGG20.11068068622025456No Hit
ATTCTGTAGGTTGCCTGTTCACTCTGATGGTAGTTTCTTTTGCTGTGCAG20.11068068622025456No Hit
GTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACT20.11068068622025456No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT20.11068068622025456No Hit
CTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGAT20.11068068622025456No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT20.11068068622025456No Hit
TTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCAC20.11068068622025456No Hit
CTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACC20.11068068622025456No Hit
GATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTAC20.11068068622025456No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG20.11068068622025456No Hit
TCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG20.11068068622025456No Hit
GTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTG20.11068068622025456No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT20.11068068622025456RNA PCR Primer, Index 33 (96% over 27bp)
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC20.11068068622025456No Hit
CGCCACCTGTTCGCGGCCGATCAGGATCAGTAGCGGCACCGCGAGCGGGC20.11068068622025456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA20.11068068622025456No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC20.11068068622025456No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC20.11068068622025456No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC20.11068068622025456No Hit
CGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGT20.11068068622025456No Hit
TCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAC20.11068068622025456No Hit
GGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTG20.11068068622025456No Hit
ACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA20.11068068622025456No Hit
TCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAG20.11068068622025456No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG20.11068068622025456No Hit
GGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGT20.11068068622025456No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC20.11068068622025456No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA20.11068068622025456No Hit
ATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATGAAAATTAT20.11068068622025456No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC20.11068068622025456No Hit
GTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGC20.11068068622025456No Hit
ATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTG20.11068068622025456No Hit
GATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT20.11068068622025456No Hit
CCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTT20.11068068622025456No Hit
TCCCTACACTGGTAAGATAAATAGTGATTGTTAAGGATATATTTTAAATG20.11068068622025456No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA20.11068068622025456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.11068068622025456No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC20.11068068622025456No Hit
GAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATT20.11068068622025456No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT20.11068068622025456No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT20.11068068622025456No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA20.11068068622025456No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC20.11068068622025456No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)