FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001324156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497857
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT12150.24404598107488698No Hit
GTACATGGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGC8280.16631281673251555No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT7230.14522242330629076RNA PCR Primer, Index 27 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.14080348373127222No Hit
CATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCG6290.12634149966757524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.11690103784821747No Hit
ATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCGT5380.1080631586981804No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT5110.10263991467429402RNA PCR Primer, Index 27 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)