FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001324157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495016
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT10670.21554858832845808No Hit
GTACATGGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGC7020.14181359794430887No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT6440.13009680495175915RNA PCR Primer, Index 27 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.12221827173263086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.11171356077379316No Hit
CATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCG5120.10343100021009421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)