FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001324158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513069
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT13680.26663080404390055No Hit
GTACATGGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGC8240.1606021802135775No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT8210.16001746353804264RNA PCR Primer, Index 27 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.13760332430920597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.12727332970808994No Hit
CATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCG6030.11752805178250879No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT5610.1093420183250206RNA PCR Primer, Index 27 (96% over 25bp)
ATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCGT5590.10895220720799736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)