FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001324201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54032
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT603411.167456322179449TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT30605.663310630737341No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT29965.544862303819959RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT10041.8581581285164346TruSeq Adapter, Index 1 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT8261.5287237192774652No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC8201.5176191886289607No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG6601.221498371335505TruSeq Adapter, Index 1 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT6431.190035534498075No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC4640.8587503701510216Illumina PCR Primer Index 5 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4050.7495558187740599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.6644210838021913No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC2120.39236008291382884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.3701510216168197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.3479419603198105No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1820.3368374296713059TruSeq Adapter, Index 1 (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1750.3238821439147172Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.25725496002368964No Hit
TTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1080.19988155167308264No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1010.18692626591649392Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.17582173526798933Clontech SMART CDS Primer II A (100% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.15916493929523245No Hit
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC850.15731418418714838No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT740.13695587799822329TruSeq Adapter, Index 1 (96% over 27bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT700.1295528575658869Clontech Universal Primer Mix Long (96% over 25bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAGGCAT690.12770210245780278No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC660.12214983713355047Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG630.11659757180929818Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA580.1073437962688777Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC580.1073437962688777Clontech Universal Primer Mix Long (96% over 25bp)
NTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT550.10179153094462541RNA PCR Primer, Index 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)