FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001324202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53844
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT51589.579526038184385TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT28235.242924002674393No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT25424.721045984696531RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT8961.6640665626625066TruSeq Adapter, Index 1 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC7251.3464824307258003No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT6931.2870514820592824No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT5781.073471510288983No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG5721.0623282074140108TruSeq Adapter, Index 1 (95% over 24bp)
ANACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT5711.0604709902681821TruSeq Adapter, Index 1 (95% over 21bp)
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC4570.8487482356437115Illumina PCR Primer Index 5 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.6815986925191293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.6630265210608425No Hit
ANACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT2680.49773419508208894Illumina PCR Primer Index 5 (95% over 22bp)
CNTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT2630.4884481093529455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.38815838347819626No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC2080.3863011663323676No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1840.34172795483247903Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.3250130005200208No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1630.3027263947700765TruSeq Adapter, Index 1 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.21729440606195674No Hit
TTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1070.19872223460366986No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1010.18757893172869772Clontech Universal Primer Mix Long (96% over 25bp)
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC920.17086397741623952No Hit
AAACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT890.16529232597875343RNA PCR Primer, Index 1 (95% over 21bp)
TNATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC850.1578634573954387No Hit
CNTCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT790.14672015452046652RNA PCR Primer, Index 1 (96% over 26bp)
AGACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT780.14486293737463785TruSeq Adapter, Index 1 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.14486293737463785No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.131862417353837Clontech SMART CDS Primer II A (100% over 21bp)
TNTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT670.12443354877052223No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAGGCAT670.12443354877052223No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT640.11886189733303619Clontech Universal Primer Mix Long (96% over 25bp)
GNGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT630.1170046801872075No Hit
CNCATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG580.10771859445806403TruSeq Adapter, Index 1 (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC550.10214694302057797Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.10214694302057797No Hit
ACACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT540.10028972587474928TruSeq Adapter, Index 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)