FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001324226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889559
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT283073.182138565289093No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT215912.427157726468958RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT106871.201381808289276RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG85800.9645228703211366RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52740.5928780440645308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45010.5059810535332676No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT39640.4456140626984832RNA PCR Primer, Index 39 (96% over 28bp)
CNTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT35740.40177211404752244No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC32970.37063308898004516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.363101267032316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27870.3133013099749426No Hit
ANACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT27300.3068936405567253RNA PCR Primer, Index 39 (95% over 23bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG27200.3057694880272135Illumina PCR Primer Index 11 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT26480.2976755898147284RNA PCR Primer, Index 39 (95% over 22bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC15500.1742436420743312Illumina PCR Primer Index 11 (96% over 26bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA13660.15355923553131384RNA PCR Primer, Index 39 (96% over 30bp)
ANACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT13640.15333440502541149Illumina PCR Primer Index 11 (95% over 24bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.14636465934243822No Hit
TNTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG11100.12478093077581139RNA PCR Primer, Index 39 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.11668703256332631Clontech SMART CDS Primer II A (100% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA9730.10938004112149953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.10701932080952473No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC9000.10117372765606329RNA PCR Primer, Index 39 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)