FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001324241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056052
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT64450.3134648345469862No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT44590.21687194681846567RNA PCR Primer, Index 23 (95% over 23bp)
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA36860.17927562143369916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.17606558588985102No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC32840.15972358675753337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32030.15578399768099252No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC30240.14707799219085899No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC25700.12499683860135832No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25190.12251635659020296Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA24910.1211545233291765No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG22910.11142714289327313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.11089213696929845No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT22560.10972485131699003RNA PCR Primer, Index 23 (96% over 25bp)
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC22260.10826574425160454No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC21330.10374251234890947No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG21090.10257522669660105No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT20770.10101884582685652No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG20760.10097020892467701No Hit
TTCTAGGATAGTCAGTAGGATTAGAATTGTGAAGATGATAAGTGTAGAGG20700.1006783875115999No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTG20600.10019201848980473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)