FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001324242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066408
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT74540.3607225678568801No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT50440.24409506738262723RNA PCR Primer, Index 23 (95% over 23bp)
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA41890.20271892094881552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39990.19352422174130182No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC38810.18781382960189857No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC34400.166472448809722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33720.16318171435650655No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA28760.13917871010952337No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28660.13869477857228582Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC27800.13453296735204276No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT26490.12819346421423067RNA PCR Primer, Index 23 (96% over 25bp)
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC25410.12296700361206499No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG25350.12267664468972245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.12262825153599871No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT24740.11972466231257332No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG24020.11624035524446284No Hit
TTCTAGGATAGTCAGTAGGATTAGAATTGTGAAGATGATAAGTGTAGAGG23800.11517570586254021No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG23610.11425623594178884No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC23290.11270765502262865No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT22580.10927174110824193No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTG22510.10893298903217564No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA22090.10690047657577788No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA21960.10627136557736905No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGT21850.10573904088640772No Hit
GTGTATGGTTATTATTTGTTTTGAGGTTAGTTTGATTAGTCATTGTTGGG21790.10544868196406519No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT21090.10206116120340222No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC20900.10114169128265087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)