FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001324249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576657
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT66660.42279328985315134No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT45070.28585798940416335RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26210.1662378056863351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22330.1416287753138444No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT22220.1409310966177171RNA PCR Primer, Index 23 (96% over 25bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG18050.1144827315008908RNA PCR Primer, Index 23 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)