FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001324253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971382
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT62410.31657994239574067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46390.23531715314434237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40960.20777302420332538No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT38030.19291035425909336RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.13548870792165088No Hit
GTACATGGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGC19910.1009951394503957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)