FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001324259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612268
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT91490.5674614890328407No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT65700.4075004899929788RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT28470.17658354566362416RNA PCR Primer, Index 31 (95% over 24bp)
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA26840.16647356394842544No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC26800.16622546623762302No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC25140.1559294112393225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24900.15444082497450795No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT23620.14650169822883044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22040.13670183865213476No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA20710.1284525897679542No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC20350.1262197103707324No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTG19800.12280836684719909No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC19540.12119573172698335No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT18190.1128224339874016No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA17780.11027943245167676No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT17670.1095971637469701No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC17650.10947311489156888No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTGT17300.1073022599220477No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG17210.10674404007274224No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC17190.10661999121734105No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC17010.10550355151873014No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT16580.10283650112760409No Hit
TAATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAA16560.10271245227220288No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG16430.10190613471209502No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG16400.10172006142899319No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT16390.10165803700129258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)