FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001324261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574357
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT55160.9603783013004108No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT37910.6600424474673418RNA PCR Primer, Index 31 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24130.42012197988359157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22380.38965312514690337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.37833612195899063No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT18460.32140289053672194RNA PCR Primer, Index 31 (95% over 24bp)
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC14880.2590723191325256No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.2588982113911731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.2541973023746555No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTG12740.22181326248308977No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTGT12390.21571949153575215No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC11980.2085810741402995No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT11320.19708996321103428No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC10330.17985329681713638No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA9980.17375952586979876No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT9480.16505413880217357No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT9460.16470592331946857No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA8840.15391124335561332No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT8570.14921033433909572No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT8540.1486880111150382No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG8010.1394603008233555No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT7770.13528171503089542No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC7700.13406296084142788No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA7570.13179956020384534No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA7540.13127723697978783No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG7290.12692454344597526No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG7280.12675043570462272No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT7200.1253575737738027No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA7100.12361649636027766No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT7010.12204952668810513No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA6930.1206566647572851No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT6860.11943791056781758RNA PCR Primer, Index 31 (96% over 27bp)
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC6600.11491110929265247No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC6260.10899144608666736No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTT6230.10846912286260983No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT6160.1072503686731423No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA5990.10429053707014975No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG5940.10341999836338722No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.10098248998445217Clontech SMART CDS Primer II A (100% over 21bp)
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC5760.10028605901904217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)