FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001324273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471390
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT96650.6568618789036218No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT68490.46547822127376154RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.1825484745716635No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT26210.1781308830425652RNA PCR Primer, Index 31 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.15685848075629166No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC15900.10806108509640543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.10568238196535248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)