FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001324321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313588
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.20504947293986656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.18685262890367688No Hit
GTACATGGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGC36720.15871451615412943No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36510.15780683509769242Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT34400.1486868016258729No Hit
ATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCGT26920.11635606685373541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26550.11475681927810828No Hit
CATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCG23770.1027408510071802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)