FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001324323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284597
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46880.2052003044738306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42650.1866850039634999No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37810.16549964829683309Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGC36830.16121005148829312No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT34370.15044228807093768No Hit
ATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCGT26990.11813899781887133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.11209854517011096No Hit
CATGTGGACCAGGCTGGCCTCGAACTCGTGCCCTGGAACCCCCGCCTCCG24930.10912209024173627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)