FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001324325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235606
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.7160259076593974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.6646689812653328No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT14930.6336850504656077TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT13590.5768104377647428No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT11530.48937633167236827RNA PCR Primer, Index 3 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.4740965849766135No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG8900.37774929331171536TruSeq Adapter, Index 3 (96% over 28bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT6860.29116406203577155TruSeq Adapter, Index 3 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.18547914739013438Clontech SMART CDS Primer II A (100% over 21bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG3870.16425727697936385TruSeq Adapter, Index 3 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.13157559654677725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)