FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001324332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581878
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT280341.7721973502381347No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT230981.4601631731397744TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT146350.9251661632565849RNA PCR Primer, Index 3 (95% over 23bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG94020.594356834092136TruSeq Adapter, Index 3 (96% over 28bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT70460.44541993756787823TruSeq Adapter, Index 3 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67800.42860448150868774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55050.3480040812249744No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG45890.29009822502114574TruSeq Adapter, Index 3 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41770.26405323292946736No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35630.22523860879283988Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC32620.2062105927258613No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT26800.16941888059635446No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC25610.16189617656987454No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC21910.138506256487542Illumina PCR Primer Index 12 (95% over 24bp)
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA21700.13717872048286908No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21570.13635691247997633Clontech Universal Primer Mix Long (96% over 25bp)
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT20150.12738024044837845No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG19140.12099542442590389No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17910.1132198563985339Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17520.1107544323898556No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA17470.110438352388743No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT17320.1094901123854052No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA17050.10778328037939713No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG16760.10595001637294404No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA16540.1045592643680486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)