FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001324339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017199
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83330.2761833077632599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70080.23226840523280037No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT58060.19243013139007406No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT55210.18298428443069217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50090.1660149032264693No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA49720.1647886002878829No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC48790.1617062712800846No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC48270.15998281850153073No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTG46470.1540170204219211No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC45180.14974153179820093No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT39340.13038583136213422TruSeq Adapter, Index 3 (95% over 21bp)
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC38100.1262760593517365No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTGT37420.12402231341055063No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA37220.12335944695726068No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG35290.11696278568301263No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA34990.11596848600307769No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC33880.11228957718731844No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG33470.11093070095807402No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC33370.11059926773142904No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT32430.10748379540096627No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT31240.10353974000389102No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGT30250.10025855106010574No Hit
TAATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAA30230.10019226441477676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)