FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001324341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002560
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82210.273799690930406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70340.23426675903229244No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT59500.19816423318767987No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT56760.18903868698710435No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA48630.1619617926036449No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC48410.1612290845145476No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC48320.1609293402962805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.1555339443674731No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTG46090.1535023446658851No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC44050.14670814238516466No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT40510.13491820313332623TruSeq Adapter, Index 3 (95% over 21bp)
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC38090.12685841415325588No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA36790.12252877544495364No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTGT36780.12249547053181284No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG35230.11733320899499093No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC34420.11463551103058722No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG33830.11267052115528083No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA33610.11193781306618351No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC32810.1092734200149206No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT32090.10687546626878398No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT31270.10414446339123946No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA30380.10118032612170948No Hit
GTATAAGAGATCAGGTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGT30340.10104710646914633No Hit
TTCTAGGATAGTCAGTAGGATTAGAATTGTGAAGATGATAAGTGTAGAGG30080.10018117872748589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)