FastQCFastQC Report
Wed 31 Aug 2022
EGAF00001324368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATTCTGTGGTTTGTCTGTATACTCTGTTGATTATTATTATTTTTT12.7777777777777777No Hit
GTACATGGGGTCCTTGGAAAAAGGTTTTCATCTCCGGTTTACAAGACTGG12.7777777777777777No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG12.7777777777777777No Hit
CTGAAGAACATCACCTAAGAGATTATTTTGAACAGTATGGAAAAATTGAA12.7777777777777777No Hit
AGAAAAATGTTTAGATGTTCCCAGAAAAGATTTAAATTGTAAACCTAAAC12.7777777777777777No Hit
CAACAATTACATCATAAGTTCTCAAGCAGAGGCAGTCTATTGCAAGGACC12.7777777777777777No Hit
CCCAGCACCACCGCCTCGCACGTGTTCCTGATCAGCCGCTCCCGGCTCTT12.7777777777777777No Hit
TGATTGTGTCGTGGTGGGGACCGTGGTTGTCATGATCATAGCCGTGTGTT12.7777777777777777No Hit
CCAGACAGTTGTGAAGGGCTCGGCAAATATAAGAGCACCATTGGCTACAT12.7777777777777777No Hit
ATATTGCTAAAGCACTAGGTGGAATCATTATGAAAATTTCCATCACTCAA12.7777777777777777No Hit
AGTGAAAACAAACCTGAAAGCTAGCAGAAGATAAGAAATAGCCAAGATCA12.7777777777777777No Hit
ATATAAATACAAACATACACAAAAAGATGGAAAAACATAAATTCAGAAAA12.7777777777777777No Hit
AGCATATGGAGATAGAATAGAAAGAATGCTTCGCCTCAGTTTGAACATTG12.7777777777777777No Hit
GATTTCAGAGCATTGACCGTAGTATACCCCCGGTCGTGTAGCGGTGAAAG12.7777777777777777No Hit
TTAGTAAAGAAGCACAAATCATGTCATAAATGTGTTTTTAAAAAATATCT12.7777777777777777No Hit
TGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGT12.7777777777777777No Hit
TCCCTACACCTTCCAACGTATTCTACTTTGCCATGAGAAAAAAACTAAAT12.7777777777777777No Hit
TACTAGTCCACTAGAAATAAGTATGTTAAACAGAAGCAACTTAACGTAAC12.7777777777777777No Hit
GATCTTATGAGGATGCTCAACAGTTAACAGTACATGTTTAGAGAGAACCA12.7777777777777777No Hit
CTCGTAAAGAGTTCTACTGGTTAATTTAAAAGTGAGAGAATCCCAGTGGA12.7777777777777777No Hit
ATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTC12.7777777777777777No Hit
CTAATTGCTATGGACACTTGAAATCTATTAAATAGCCTTCGCATAGTGGT12.7777777777777777No Hit
GAGTGCAGTGGTGCGATCTCGGCTCACTCACCGCAACCTCCACCTCCTGG12.7777777777777777No Hit
TCTTACAGTAACCTACTTGCAGTTGTATTTAACTGAGCTCTGTTGCTGTG12.7777777777777777No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT12.7777777777777777RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12.7777777777777777No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT12.7777777777777777No Hit
CCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTC12.7777777777777777No Hit
GAAGCAGTTTATGAGACAGCTGAGTGCCCTGTCCTCAGATGGGGAGGGAC12.7777777777777777No Hit
AGCCGAGATTGCGCCACTGCACTCCAGCCTGGGTAACAGAGCAAGACTCC12.7777777777777777No Hit
GTGGTGTGATCTCGACTCACTGCAACCTCCACCTCCACCTTCTGGGTTCA12.7777777777777777No Hit
GTTCATTAACTCTGCATTAACTATAGAAAGGTTGCTAGTTAATGCTTATT12.7777777777777777No Hit
CGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCA12.7777777777777777No Hit
GGCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGACATAACTA12.7777777777777777No Hit
GAGTACATGGGGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAA12.7777777777777777No Hit
ATCTCCGGGGTGGCCACAGGCTAGATCCCCGGTGGTTTTGTGCTCAAAAT12.7777777777777777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)