FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001324494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001324494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG120.7518796992481203RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG100.6265664160401002RNA PCR Primer, Index 26 (100% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT50.3132832080200501RNA PCR Primer, Index 31 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2506265664160401No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT40.2506265664160401RNA PCR Primer, Index 27 (96% over 28bp)
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT30.18796992481203006No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT30.18796992481203006RNA PCR Primer, Index 31 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18796992481203006No Hit
CTTCCAAGTCAACCTCCTCAGAGAGGTTCATCTTGCTAGTGATAGTGGAG20.12531328320802004No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG20.12531328320802004RNA PCR Primer, Index 39 (96% over 30bp)
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA20.12531328320802004No Hit
GTATCAACGCAGAGTACATGGGGTTAACGCGTCACGGGCGGCCTGGCAGC20.12531328320802004No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA20.12531328320802004RNA PCR Primer, Index 1 (96% over 28bp)
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA20.12531328320802004No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG20.12531328320802004RNA PCR Primer, Index 23 (96% over 30bp)
CCTAAATGATGGTCGGGCACCAATAAAGTGCCATCCATATCAAATGCTGC20.12531328320802004No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12531328320802004Clontech SMART CDS Primer II A (100% over 21bp)
TATATATATATTCTACTTCCAACACCCGCATTCATCCTGGTTCAATCAAA20.12531328320802004No Hit
CCACAGCTCGTCTCTTCATCTTGGTTTTGCCACAGAAAGAGCAAGTGTAC20.12531328320802004No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG20.12531328320802004RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG20.12531328320802004TruSeq Adapter, Index 10 (96% over 28bp)
CATTTGTATGAGTCATGATTTTATCTTTAAAAAATTATTCTTTGGCCAGG20.12531328320802004No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA20.12531328320802004RNA PCR Primer, Index 31 (96% over 29bp)
GAAAGAGCAAGTGTACTTGGCGTGCTGGCTGATTTCAATTTTCTTCACCA20.12531328320802004No Hit
CAGCGGAAGTGGTATTGTACGTCCAGGCACCGCCAGCCACTGTCTTCATG20.12531328320802004No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG20.12531328320802004TruSeq Adapter, Index 3 (96% over 28bp)
AGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCC20.12531328320802004No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA20.12531328320802004RNA PCR Primer, Index 26 (100% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)