FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001327703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001327703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489100
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1129574.5380659676188175Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT832313.343819051062633Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT520402.0907155196657428Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC484131.9450002008758187Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT357111.4346952713832308Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT292001.1731147804427304Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC271621.091237796794022Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC223390.8974729822024025Clontech SMARTer II A Oligonucleotide (95% over 24bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN213550.857940621108031No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA199620.8019766180547185Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC193980.7793178257201397Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG173710.6978827688722832Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA173090.695391908722028Clontech Universal Primer Mix Long (96% over 25bp)
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG156790.6299063918685468Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC142370.5719738057932586Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC136090.5467438029809971Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT133160.5349724800128561Clontech SMART CDS Primer II A (96% over 26bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC128160.5148848981559601Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA121130.4866417580651641Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG113970.45787634084608897Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC113240.45494355389498214Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT104550.42003133662769676Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC99660.4003856815716524Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG97380.3912257442449078Clontech Universal Primer Mix Long (96% over 25bp)
GTACATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA92250.37061588525973244Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT87390.35109075569482945Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG69390.2787754610100036Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCA56770.22807440440319793Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG55460.22281145795669116Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA52290.21007593105941907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT49570.1991482865292676Clontech Universal Primer Mix Long (96% over 28bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT49330.19818408260013662Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT48990.19681812703386767Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA48940.19661725121529872Clontech Universal Primer Mix Long (95% over 24bp)
CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC48890.19641637539672976Clontech SMART CDS Primer II A (96% over 26bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA47140.18938572174681612Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC47070.18910449560081957Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACT44500.178779478526375Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.1745610863364268No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA38330.15399140251496524Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA36380.14615724559077578Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT36060.14487164035193442Clontech Universal Primer Mix Long (96% over 28bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA35440.14238078020167932Clontech Universal Primer Mix Long (96% over 25bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35390.14217990438311034Clontech Universal Primer Mix Long (96% over 27bp)
TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC33830.1359125788437588Clontech SMART CDS Primer II A (96% over 26bp)
CTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG31830.12787754610100036Clontech SMART CDS Primer II A (96% over 26bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG31680.12727491864529347Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.11948093688481781No Hit
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTA28340.1138564139648869Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)