FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001327986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001327986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687478
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85430.5062584519620404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68710.4071756787347746No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT61720.36575291648246677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37690.22335105998418942No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT31790.18838764120184087RNA PCR Primer, Index 31 (95% over 22bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG31710.18791356094716494No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20790.12320160618390283Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA19960.11828302354164025No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT19510.11561632210908823RNA PCR Primer, Index 31 (95% over 24bp)
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT19150.11348296096304664No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA18480.10951253883013587No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA18200.10785325793877015No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)