FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001348174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16678757
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC469951028.17662011623528TruSeq Adapter, Index 4 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC307130.18414441795632613TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCCACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC198450.11898368685388246TruSeq Adapter, Index 4 (100% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)