FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001348251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301713
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG1099294.775964683694275No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT506242.199405399369948No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT487882.1196387212480445No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC400561.740269095234723No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA311291.3524275181136831No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA310941.3509069115046055No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT304681.3237097761536734No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA274841.1940672012540225RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT267071.1603097345324982No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA222850.9681919509513132No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC202810.8811263611058372No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA188530.8190856114554681RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC179410.7794629478132156RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC171180.743706969548332RNA PCR Primer, Index 1 (100% over 26bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC153360.6662863701947201No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT150480.6537739500971668No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA143520.6235356015280793No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT143110.6217543195003026No Hit
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT128430.5579757337252733No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA121470.5277373851561858No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC112640.4893746527043119No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGG112540.4889401936731469No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG111840.4858989804549916No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG108510.4714314947171954No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC103400.44923063822466136RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG101470.44084557892317594No Hit
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAA98740.4289848473723701No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT95650.41556006330937006No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA95160.4134312140566612No Hit
TAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTG92150.40035399721859327No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT91400.39709555448485545No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAA90570.3934895445261855No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA86890.3775014521793117No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT86450.37558983244218547No Hit
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT74390.3231940732836805No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATTC71190.30929138428639885No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC70680.3070756432274571No Hit
ATCGCTGTACGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGAT69310.30112355450049594No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC66720.28987106559332115RNA PCR Primer, Index 1 (100% over 27bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC65790.2858305966034862No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG63990.2780103340425153No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC62600.2719713535093211No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA62530.27166723218750555No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC61900.26893014029116574No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG59870.26011062195851525No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT59250.25741697596529195No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG57740.2508566445946997No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC57100.2480761067952434RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT56320.24468732635215595No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC55890.24281915251814626No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC55670.24186334264958315No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC55520.2412116541028356No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA55460.24095097868413656No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA52490.22804754545853456RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC52270.22709173558997145No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTG52090.22630970933387437No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCT51340.22305126660013652No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG49720.2160130302952627No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA48150.2091920235059714RNA PCR Primer, Index 1 (100% over 28bp)
CTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTCTCGG47940.20827965954052483No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA47630.20693283654391317No Hit
ACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTC46880.20367439381017532No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA46620.20254480032914618No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT46450.20180621997616557No Hit
AATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAAT46320.201241423235651No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGG46140.20045939697955392No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT45970.19972081662657332RNA PCR Primer, Index 1 (100% over 25bp)
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGA45110.1959844689585539No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC41790.18156042912387427No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG41760.18143009141452474No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC40770.17712894700599074No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC40000.1737836124660199No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG39860.1731753698223888No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT38740.16830942867334026No Hit
CTGGTATTGCAGTACTTCCAGGAACGGTGCACCATGGAATTCTCGGGTGC38510.16731017290166064No Hit
TAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTT38160.16578956629258296No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG38150.16574612038946646No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC37950.16487720232713635RNA PCR Primer, Index 1 (100% over 27bp)
AATCGCTGTACGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGA37910.16470341871467034No Hit
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT37360.16231389404326255No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG36530.15870788408459266No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG36200.157274169281748No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC35860.15579700857578682RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA35440.15397228064489363RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA34460.14971458213947614RNA PCR Primer, Index 1 (100% over 22bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC34320.14910633949584504No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATGGAATTCTC32970.1432411425751169No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC32640.1418074277722722No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA32350.14054749658189358No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAATGGAATT32290.14028682116319455No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC32050.13924411948839843No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC31840.13833175552295182RNA PCR Primer, Index 1 (100% over 26bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCG31630.1374193915575052No Hit
TTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTC31530.13698493252634017No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG31370.1362897980764761Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG31200.1355512177234955No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA31150.13533398820791298No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC30320.13172797824924307No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA30250.13142385692742753No Hit
AACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGT30190.1311631815087285No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG30070.13064183067133045No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGGG29050.12621034855344693No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG28940.12573244361916538No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATATGGAATTC28570.1241249452038547No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG28550.1240380533976217No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG27330.11873765321740808No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCTC27140.11791218105819448No Hit
ACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGG26880.11678258757716535No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC26840.11660880396469935RNA PCR Primer, Index 1 (100% over 24bp)
CTCTACTGAACTGCCATGAGGAAACTGCCATGTCACCCTTCTGACTTGGA26780.11634812854600031No Hit
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG26430.11482752193692264No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAAT26350.1144799547119906No Hit
TTTCTGATGGATTAGTGGAGAAAACAGAAAATTCTGAGTTGGAATTCTCG26290.11421927929329156No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG26000.11295934810291292Illumina Small RNA Adapter 2 (100% over 21bp)
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTGGAATTCT25840.11226421365304884No Hit
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG25450.11056982343150515No Hit
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC25210.10952712175670903RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG25050.10883198730684494No Hit
CACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGG25000.10861475779126242No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA24970.1084844200819129RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA24800.10774583972893233RNA PCR Primer, Index 1 (100% over 22bp)
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG24430.10613834131362164No Hit
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG24380.1059211117980391No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG24370.10587766589492262No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTTGGAATTCTC23950.1040529379640294No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGC23700.10296679038611677No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTGGAATTC23620.10261922316118473No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTT23610.10257577725806824No Hit
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC23610.10257577725806824RNA PCR Primer, Index 1 (100% over 24bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA23480.10201098051755367No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)