FastQCFastQC Report
Sat 13 May 2023
EGAF00001348252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13804698
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT5070393.6729452538548832TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA3656992.6490909109348135TruSeq Adapter, Index 16 (97% over 40bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG997690.7227177298626888Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTATGCCGTCTTCTGCTTGGATCGGAAGAGCACACGTCTGAACTCCAGT850350.6159859491312305Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTAT392950.2846494722303958TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTA304180.2203452766587143TruSeq Adapter, Index 16 (97% over 40bp)
CGTATGCCGTCTTCTGCTTGCACACGTCTGAACTCCAGTCACCCGTCCCG206040.14925353673075645TruSeq Adapter, Index 16 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)