FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001348260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33418764
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC380794911.394643440433644RNA PCR Primer, Index 1 (100% over 50bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC29637828.868616445539399RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC17126955.124950162728939RNA PCR Primer, Index 1 (100% over 27bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4969311.4869819841332252RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC4892091.4638752049597046RNA PCR Primer, Index 1 (100% over 36bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC4597821.3758198837036582RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC4288431.2832401581339155RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC4100321.2269514216623931RNA PCR Primer, Index 1 (100% over 26bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4090501.2240129527232066RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC3610641.0804229623812538RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3321400.9938727835655442RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC3108680.930219920760684RNA PCR Primer, Index 1 (100% over 27bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA2615120.7825304370921677No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2505220.7496447205528008RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2503420.7491061009916464RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT2454040.7343299710306461RNA PCR Primer, Index 1 (100% over 25bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2390550.7153316621763749RNA PCR Primer, Index 1 (100% over 34bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT2388430.714697288026571No Hit
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2191470.6557603387127064RNA PCR Primer, Index 1 (100% over 28bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2048830.6130777308221214RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1920500.5746771484427132No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA1833610.5486767852934358RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1774320.5309352554151913RNA PCR Primer, Index 1 (100% over 35bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG1750720.523873354502279RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1713440.5127179449245939RNA PCR Primer, Index 1 (100% over 32bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC1614810.4832045853042321RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1597360.4779829678919304RNA PCR Primer, Index 1 (100% over 31bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1541090.46114512194406715RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1510300.45193173511743284No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1455330.43548289218595876RNA PCR Primer, Index 1 (100% over 33bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1402950.4198090629563679RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1382020.41354611439250116No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1290460.3861483327151178RNA PCR Primer, Index 1 (100% over 29bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1261310.37742568815531297RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1260240.3771055087495157RNA PCR Primer, Index 1 (100% over 28bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC1189790.3560245375921144RNA PCR Primer, Index 1 (100% over 24bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1141900.34169426493451405RNA PCR Primer, Index 1 (100% over 23bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC1134130.33936922382886453RNA PCR Primer, Index 1 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1126350.3370411903923197No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1084670.3245691552207018RNA PCR Primer, Index 1 (100% over 28bp)
TAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG1060460.3173247221231761RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1056900.31625945232444863RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1038220.3106697782120248RNA PCR Primer, Index 1 (100% over 31bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA963620.2883469897330733RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG945950.283059541041075No Hit
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC927280.2774728592595465RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG916480.27424114189262055Illumina Small RNA Adapter 2 (100% over 21bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT888560.2658865540329379No Hit
AGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCA878980.26301990103523876RNA PCR Primer, Index 1 (100% over 28bp)
CAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC813710.24348895728160386RNA PCR Primer, Index 1 (100% over 27bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG801490.23983232892754502No Hit
TACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT798530.2389465989825357RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC792680.2371960854087841RNA PCR Primer, Index 1 (100% over 27bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT780430.23353048006203941RNA PCR Primer, Index 1 (100% over 25bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA762890.22828193167168RNA PCR Primer, Index 1 (100% over 34bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA740510.22158509512799457RNA PCR Primer, Index 1 (100% over 23bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC740180.22148634820844962RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA731290.2188261660425263RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC727380.2176561646624633No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA700670.20966364884111213RNA PCR Primer, Index 1 (100% over 22bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA700290.20954994026709067RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC697110.2085983790423847RNA PCR Primer, Index 1 (100% over 27bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA690560.20663840230596198RNA PCR Primer, Index 1 (100% over 28bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC683040.20438816947269506RNA PCR Primer, Index 1 (100% over 33bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA652870.19536030716156946RNA PCR Primer, Index 1 (100% over 23bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT649990.19449851586372255RNA PCR Primer, Index 1 (100% over 35bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT627610.1878016793200371RNA PCR Primer, Index 1 (100% over 30bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA624320.1868172024554828RNA PCR Primer, Index 1 (100% over 34bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA611410.18295410326964814RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA601900.18010839658821615RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA584500.17490174083039098RNA PCR Primer, Index 1 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC571840.1711134499169389No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC518770.15523314985557216RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG518050.15501770203111043No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC509440.15244130513025556No Hit
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT491580.14709700215124652RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA479600.14351218973867494RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG476410.14255763618307368RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT476040.14244691993994751RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT474140.14187837706984016RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA457010.13675251424618814RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC449590.13453220472187422RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA422680.12647984228261702RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC418380.12519313999763726RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA405460.1213270484809073RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA401490.12013909311547251RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA401180.12004633085771813RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC399850.119648350848643RNA PCR Primer, Index 1 (100% over 26bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC390840.11695226071197606RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC387390.11591990655309695RNA PCR Primer, Index 1 (100% over 27bp)
GTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC363180.10867547345557124RNA PCR Primer, Index 1 (100% over 31bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC360360.10783163614309613RNA PCR Primer, Index 1 (100% over 31bp)
TACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT355880.10649107190200092RNA PCR Primer, Index 1 (100% over 35bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC354040.10594048301726539RNA PCR Primer, Index 1 (100% over 36bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC353950.10591355203920767RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC343910.10290925182032465No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA335890.10050940244229259RNA PCR Primer, Index 1 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC334690.10015032273485637RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG334220.1000096831827772RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph