FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001348294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27728714
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA13430894.843675765129245RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC13376244.82396695353416RNA PCR Primer, Index 1 (100% over 27bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA11248214.056520616138203No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT8310592.9971061766513944No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC5822872.0999423197195513RNA PCR Primer, Index 1 (100% over 26bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA5184811.869834280810859RNA PCR Primer, Index 1 (100% over 23bp)
TCTCCCAACCCTTGTACCAGTGTTGGAATTCTCGGGTGCCAAGGAACTCC4595901.6574515500430347RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC3626291.3077743165442146RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3401751.2267968864333196RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3258301.1750635099774192RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA2924631.0547297649649385RNA PCR Primer, Index 1 (100% over 22bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC2611320.9417385891029781RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT2558720.9227690833408285No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2456600.8859408337508909No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG2446760.8823921657528004No Hit
TCTCCCAACCCTTGTACCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2234430.8058181133102674RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA2175700.7846379027891448No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2063980.744347538079119No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1901170.685632229464374RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1890540.6817986582428598RNA PCR Primer, Index 1 (100% over 26bp)
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG1839150.6632655232406378No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1799150.6488400435736038RNA PCR Primer, Index 1 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1655350.5969804441706168RNA PCR Primer, Index 5 (100% over 50bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1625110.5860747815423392RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1486640.5361373773049843RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1463010.527615525191684No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1417260.5111163828225139RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1336260.48190478649677004No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1264820.4561408798114474RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA1208330.4357684961516787RNA PCR Primer, Index 5 (100% over 36bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC1192770.4301569845612025RNA PCR Primer, Index 1 (100% over 24bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC1178780.42511167304765735RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1171270.4224032892401718No Hit
TCTCCCAACCCTTGTACCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1141080.41151565846147786RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1118510.40337608155935395No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1026650.37024796750401046RNA PCR Primer, Index 1 (100% over 28bp)
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG1024740.3695591508499096No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT1023930.36926703488665213RNA PCR Primer, Index 1 (100% over 25bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC1011200.36467612598261856No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA880830.3176598813778382RNA PCR Primer, Index 1 (100% over 28bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT869550.31359189611173455RNA PCR Primer, Index 1 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT856870.3090190190572848No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC852660.3075007373223295RNA PCR Primer, Index 1 (100% over 26bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA814000.29355851122414117No Hit
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC801550.28906858067777685No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC772150.2784658531225069No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG771410.27819898174866675No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT750190.2705462647853052RNA PCR Primer, Index 1 (100% over 30bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC725620.2616854138998296No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC722020.26038712072979653RNA PCR Primer, Index 1 (100% over 26bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC717140.2586272122104184No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT709190.2557601481265954No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA704430.2540435160462184No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG701810.25309864712802765Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC693630.2501486365361192No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA680130.24528003714849525RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA652050.23515335042223737RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA641190.23123683269263764RNA PCR Primer, Index 1 (100% over 22bp)
GTCCATGATGATTTCAAGTTATCCCTGTCTGAAGTGGAATTCTCGGGTGC606840.21884895202857224No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG605880.21850274051656346Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC577460.20825343721313583No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT572170.20634566752717057RNA PCR Primer, Index 1 (100% over 25bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC568910.20516999093430732RNA PCR Primer, Index 1 (100% over 33bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA561770.20259504281374174RNA PCR Primer, Index 1 (100% over 32bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC536560.1935033842535936RNA PCR Primer, Index 1 (100% over 31bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG530330.19125661579545303No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA528570.19062189469010354RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA527500.1902360131090104RNA PCR Primer, Index 1 (100% over 28bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA501810.18097124879285784RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC494880.17847203444054419RNA PCR Primer, Index 1 (100% over 26bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG493980.17814746114803592RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC472900.17054523336350905No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT469220.1692180892341419No Hit
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG465290.16780078585685582No Hit
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG463550.16717327749133984RNA PCR Primer, Index 1 (100% over 29bp)
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA455000.16408983121251133No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG454550.16392754456625722No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT452540.16320266421298874No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC449030.16193682837220652RNA PCR Primer, Index 5 (100% over 35bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA448010.16156897864069714RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC447740.16147160665294466RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA442810.15969366628398274RNA PCR Primer, Index 1 (100% over 23bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC430100.15510997011978267RNA PCR Primer, Index 1 (100% over 33bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG420310.15157933397127613No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG418980.15109968677234725Illumina Small RNA Adapter 2 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC418700.15099870841467802RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT412120.14862571700945093No Hit
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG410340.1479837831642679No Hit
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT383700.1383764137060233No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC374680.13512346804110714No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT370190.13350420794848256RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG369610.1332950384933106RNA PCR Primer, Index 1 (100% over 29bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG356210.1284625028048542No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA354940.12800449382542586No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC354450.1278277816995047No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG344750.12432960288024898Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA342300.12344604225064315RNA PCR Primer, Index 1 (100% over 23bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC339240.12234249305611504RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC335630.12104059351616522RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC331990.11972787486646513RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA327320.1180437001153389No Hit
TCTCCCAACCCTTGTACCAGTGAATGGAATTCTCGGGTGCCAAGGAACTC325930.11754241469690949RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGG324130.11689326811189296Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA322780.11640640817313058RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG320160.11546153925493985RNA PCR Primer, Index 1 (100% over 29bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCG316240.11404784224757052No Hit
CTGGTATTGCAGTACTTCCAGGAACGGTGCACCATGGAATTCTCGGGTGC315970.11395047025981803No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGTGGAATTCTCGGGTGCC301160.10860943641309873No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCG298550.10766817386482475No Hit
GTTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACA298000.10746982351940303No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT293470.10583613794711143No Hit
ATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGTGC285940.10312054139979228No Hit
CTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGAT284430.10257597954236176No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC282700.10195207754676253No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG282490.10187634377851061No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA281910.1016671743233386No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA281140.1013894838397482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)