FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001348303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649088
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC4817733.8087568052337053RNA PCR Primer, Index 6 (100% over 50bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT3714722.93674927393975No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG2301011.8191113857378494No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT1876141.4832215571589034No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA1655081.3084579694599328No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1492211.179697698363708RNA PCR Primer, Index 1 (100% over 23bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA1225180.9685915696056505No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC1131880.8948313111585594No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1131620.8946257627427368RNA PCR Primer, Index 5 (97% over 35bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC1091690.8630582694973741RNA PCR Primer, Index 6 (100% over 44bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1055560.8344949454063407No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC930820.735879140061323RNA PCR Primer, Index 1 (100% over 26bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC881860.6971727922202771No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA865410.6841679020653505RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC839680.6638265146072191No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT802910.6347572251849304No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA800970.6332235177745621No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC795620.6289939638335981RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC675140.5337459902247498RNA PCR Primer, Index 1 (100% over 27bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA588330.4651165364649214No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC586940.4640176430111009RNA PCR Primer, Index 1 (100% over 33bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA555340.43903560478036047No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC536220.42391989050910234No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT522060.4127254075550743No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA469180.37092002206008845RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG463480.3664137683285941No Hit
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG434280.34332910009006185No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC422940.3343640268768784RNA PCR Primer, Index 1 (100% over 31bp)
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG405980.32095594559860763No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG386780.3057769856609425No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC378700.2993891733538418No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA371690.29384727183493387RNA PCR Primer, Index 1 (100% over 28bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC359870.28450272462330883RNA PCR Primer, Index 6 (100% over 36bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC358040.28305598000425014RNA PCR Primer, Index 6 (100% over 36bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA348620.2756088027848332No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG327410.25884079547869376No Hit
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA314270.24845269477135426RNA PCR Primer, Index 6 (100% over 37bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT311800.24649998482104007No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC300710.23773255431537832RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT291720.2306253225528987RNA PCR Primer, Index 1 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG291470.230427679845377No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA290860.22994543163902412RNA PCR Primer, Index 1 (100% over 32bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA289160.22860146122787667RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA287530.2273128307748353RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA287420.22722586798352576No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCT286410.22642739144513815No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG284550.22495692970117687No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG271900.21495620870057983No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG270410.21377825816375062No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG269170.21279795033444307RNA PCR Primer, Index 6 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC253750.20060734813450581RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC247490.19565837473816292RNA PCR Primer, Index 5 (97% over 35bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT245160.1938163447040609No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG243260.19231426012689612RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT238300.18839302880966596No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC236260.18678026431628905RNA PCR Primer, Index 1 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA234070.18504891419839914RNA PCR Primer, Index 1 (100% over 22bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC230200.1819894050859635RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC225990.1786611018912984No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA210890.1667234823569889RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC207620.16413831574260532RNA PCR Primer, Index 1 (100% over 24bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA206960.16361653899474807RNA PCR Primer, Index 1 (100% over 28bp)
AATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAAT206940.16360072757814634No Hit
AACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGT205060.1621144544175833No Hit
ACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTC203660.1610076552554619No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC203300.16072304975663068RNA PCR Primer, Index 6 (98% over 50bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG199700.15787699476831848RNA PCR Primer, Index 6 (100% over 45bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT199640.15782956051851327No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC198120.15662789285678147No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG194600.1538450835348762RNA PCR Primer, Index 6 (100% over 34bp)
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT192960.15254854737353396No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGA188040.1486589388895073No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT187170.14797114226733185No Hit
ATCGCTGTACGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGAT185940.1469987401463252No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC184280.14568639256838123RNA PCR Primer, Index 1 (100% over 27bp)
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA183320.14492744457149798No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAATGGAATT182460.1442475536576234No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA179480.14189165258396494No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA175730.13892701197113974RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC173260.13697430202082553RNA PCR Primer, Index 1 (100% over 26bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC172730.13655529948087958No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG172260.1361837311907388No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA169340.13387526436688557RNA PCR Primer, Index 1 (100% over 22bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA168560.13325861911941794RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAA163160.12898953663694962No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA161540.12770881189220915No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGG160980.1272660922273606No Hit
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC158800.12554264781777152No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT158140.12502087106991427No Hit
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT156130.12343182370143997No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA154740.12233293024761943RNA PCR Primer, Index 6 (100% over 37bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA151360.11966080084192632RNA PCR Primer, Index 1 (100% over 23bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT149510.11819824480626588RNA PCR Primer, Index 6 (100% over 39bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT146870.11611113781483692RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC142730.1128381745782779No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATTC142080.11232430353872153No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA138020.10911458596856943RNA PCR Primer, Index 1 (100% over 28bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT137760.10890903755274689RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT136480.10789710689023588No Hit
TAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTG135260.10693261047753008No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA134900.10664800497869886RNA PCR Primer, Index 6 (100% over 38bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA130290.10300347345199906No Hit
CTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTCTCGG129560.10242635674603577No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG129320.10223661974681494No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT128340.10146186033332996RNA PCR Primer, Index 6 (100% over 39bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT128320.10144604891672822RNA PCR Primer, Index 6 (100% over 43bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA128280.10141442608352476No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA126730.10018904129689032No Hit
AATCGCTGTACGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGA126660.10013370133878427No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC126620.1001020785055808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)