FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001348304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33624475
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC646090019.214872499868026TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG18106135.384806751629579TruSeq Adapter, Index 6 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTC5398791.6056131731424803TruSeq Adapter, Index 6 (100% over 50bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC2848130.8470407344649991TruSeq Adapter, Index 6 (100% over 42bp)
AAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTT2053550.6107307251637386TruSeq Adapter, Index 6 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTG1136290.33793538783876925TruSeq Adapter, Index 6 (100% over 50bp)
GCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGC1024450.30467390197170363TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACCAGATCATCTCGTATG727970.2165000345730305TruSeq Adapter, Index 23 (97% over 36bp)
GAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCT723870.21528068467983516TruSeq Adapter, Index 6 (100% over 50bp)
CGGTGGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT603350.1794377458681511TruSeq Adapter, Index 6 (100% over 39bp)
CGGTGGTCGCCGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG572440.17024503728311Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT518900.15432211209245647TruSeq Adapter, Index 6 (100% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA504360.14999788100780756TruSeq Adapter, Index 6 (100% over 37bp)
CACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCT380260.11309024155767489TruSeq Adapter, Index 6 (100% over 50bp)
CGGTGGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA354440.10541131125467386TruSeq Adapter, Index 6 (100% over 40bp)
ACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTT341150.10145883318624306TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)