FastQCFastQC Report
Tue 19 Sep 2023
EGAF00001348305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33624475
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG418770312.454329770204591Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC17390315.171920156374188Illumina Single End PCR Primer 1 (100% over 50bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2749210.8176216877735638Illumina Single End PCR Primer 1 (100% over 43bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1457210.43337777021053864Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG1002080.2980210099934646Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG703450.2092077274068963Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG607230.18059166723049208Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG534550.15897645985550704Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG530790.15785822678272302Illumina Single End PCR Primer 1 (98% over 50bp)
GCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC513840.1528172558828056Illumina Single End PCR Primer 1 (100% over 40bp)
CGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC459670.13670696717197814Illumina Single End PCR Primer 1 (100% over 38bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCCG386710.11500848712136026Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG374660.1114247880450178Illumina Single End PCR Primer 1 (98% over 50bp)
CGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG362890.1079243616443082Illumina Single End PCR Primer 1 (100% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG340200.10117630089391731Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAACGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG336360.1000342756280953Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)