FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001348306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014322
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1272154.220352039364076RNA PCR Primer, Index 1 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1008963.3472203699538405RNA PCR Primer, Index 1 (100% over 50bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC959713.1838337111960833RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG744952.4713683541439835No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT721072.392146558994029RNA PCR Primer, Index 1 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC538371.7860401111759128RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC399561.3255385456497348RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC377601.2526863420696264RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC347901.1541567224735778RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA339861.1274840577748497RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC332041.101541242110166RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT320681.06385449198858No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA318101.0552953533165998No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT295070.9788934294345462No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA254750.8451320064677895No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC251700.8350136448594411RNA PCR Primer, Index 1 (100% over 36bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT224530.7448772891549078No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC197890.6564992061233007RNA PCR Primer, Index 1 (100% over 31bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA195690.6492007157828527RNA PCR Primer, Index 1 (100% over 37bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA181030.6005662301505944RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC166890.5536568422351693No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA166130.5511355455721054No Hit
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC159290.5284438756045307RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT148810.4936765216191236RNA PCR Primer, Index 1 (100% over 35bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC146980.48760550465411456RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG146410.4857145321568167No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC133020.44129326594836255No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC131410.4359520980173982RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG131130.4350231992467958RNA PCR Primer, Index 1 (100% over 45bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA122860.40758751055792974RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA111240.369038211577927RNA PCR Primer, Index 1 (100% over 28bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA100420.3331429090853598No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC98370.32634204308630593RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA97640.32392027129152096RNA PCR Primer, Index 1 (100% over 34bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG97130.32222834853078075No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC96950.32163119932110773RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC96340.319607526999438RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA91080.3021575000945486RNA PCR Primer, Index 1 (100% over 34bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG86420.28669797055523594RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACATCACGATCTC82570.2739256124594519RNA PCR Primer, Index 1 (97% over 44bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA80120.26579774821668023RNA PCR Primer, Index 1 (100% over 23bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG74620.24755152236556016RNA PCR Primer, Index 1 (100% over 49bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA73710.2445326013611021RNA PCR Primer, Index 1 (100% over 22bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC73050.2423430542589677RNA PCR Primer, Index 1 (100% over 38bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG72330.2399544574202756No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT69660.23109674414345913RNA PCR Primer, Index 1 (100% over 25bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC68980.22884084712913882RNA PCR Primer, Index 1 (100% over 36bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC68830.22834322278774463RNA PCR Primer, Index 1 (100% over 44bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC65590.21759453701363027RNA PCR Primer, Index 1 (98% over 50bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT63970.2122201941265731No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG62940.20880317364899967RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG61840.20515392847877564RNA PCR Primer, Index 1 (100% over 39bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA59720.1981208377870712No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT58620.19447159261684716No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA57940.19221569560252685RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC57390.19039107301741487RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA55810.18514942995472944RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA54850.18196463416980668RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT53380.17708791562414367No Hit
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT52630.17459979391717276No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAA51010.16922545103011555No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA50800.1685287769521637RNA PCR Primer, Index 1 (100% over 32bp)
GCGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC50510.1675667032254683RNA PCR Primer, Index 1 (100% over 31bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTAT50290.16683685419142347RNA PCR Primer, Index 1 (100% over 48bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA49650.16471365700147497RNA PCR Primer, Index 1 (100% over 28bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT49630.16464730708928907RNA PCR Primer, Index 1 (100% over 30bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG48280.16016868801674142RNA PCR Primer, Index 1 (100% over 39bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA47810.15860946508037296RNA PCR Primer, Index 1 (100% over 34bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT44450.14746267983314323RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC44320.14703140540393494RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGAAATTCTCGGGTGCCAAGGAACTCCAGTCACAT44270.14686553062347021RNA PCR Primer, Index 1 (97% over 35bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC44180.1465669560186337No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC43690.14494138317007937No Hit
TGTCTGAGCGTCGCTTGTAATTCTCGGGTGCCAAGGAACTCCAGTCACAT42640.14145801278032008RNA PCR Primer, Index 1 (97% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC42590.14129213799985535RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA42580.1412589630437624RNA PCR Primer, Index 1 (100% over 28bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC42280.14026371436097404RNA PCR Primer, Index 1 (100% over 33bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA42270.1402305394048811RNA PCR Primer, Index 1 (100% over 28bp)
CGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT41770.1385717916002338RNA PCR Primer, Index 1 (100% over 46bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC40870.1355860455518687No Hit
GCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTT40500.1343585721764297No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA40010.13273299932787538No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC39120.12978042823560323RNA PCR Primer, Index 1 (100% over 31bp)
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT38190.12669515731895928No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA37630.12483735977775433RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT37400.12407433578761659No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC36940.12254828780734109RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG36180.12002699114427723No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC35960.11929714211023242RNA PCR Primer, Index 1 (100% over 38bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA35220.11684219535935444RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA34620.1148516979937777RNA PCR Primer, Index 1 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC34010.11282802567210803RNA PCR Primer, Index 1 (100% over 26bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG33920.11252945106727151RNA PCR Primer, Index 1 (100% over 39bp)
AGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA33870.11236357628680678RNA PCR Primer, Index 1 (100% over 40bp)
TGAGGGGCAGAGAGCGAGACTTTTTGGAATTCTCGGGTGCCAAGGAACTC33530.11123562777964663RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGGAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC31780.10543001046338116RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTGGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC31740.10529731063900938RNA PCR Primer, Index 1 (96% over 33bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT31190.10347268805389737RNA PCR Primer, Index 1 (100% over 46bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC30610.10154854060050651RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC30430.10095139139083349RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)