FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001348310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11727796
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA3801313.2412825052550365No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA2266011.932170375405575RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC1481011.2628203969441487RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1404761.1978039181445517RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1172070.9993949417264761RNA PCR Primer, Index 1 (100% over 22bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC1019140.8689953338206087RNA PCR Primer, Index 1 (100% over 27bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT951940.8116955649637835No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG947900.8082507574313195No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC895850.7638690168212339No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT776440.6620510793332354No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG655900.559269618946305No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC619580.5283004581594019RNA PCR Primer, Index 1 (100% over 27bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC592370.5050991678231783No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA588570.5018590023223459RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC579770.49435546116252366RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA552550.4711456440749822No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC530150.4520457211227071RNA PCR Primer, Index 1 (100% over 31bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG516930.44077335588033767No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT494090.42129825587007136RNA PCR Primer, Index 1 (100% over 25bp)
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG479230.4086275034115532No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA473740.40394631693798216RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC470990.40160146032553773RNA PCR Primer, Index 1 (100% over 26bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT453640.3868075467888425No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC443190.3778970916615535No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT410660.35015956962416467No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA408970.34871854865142604RNA PCR Primer, Index 1 (100% over 28bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT398680.3399445215452247No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA388220.3310255396666177No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC384390.32775979391183135RNA PCR Primer, Index 1 (100% over 26bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG372790.3178687623829746RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA364210.31055280975214783RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT359580.30660492389192306No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA357750.30504452840073276No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC344540.29378068990968126RNA PCR Primer, Index 1 (100% over 33bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG341630.29129940527614906No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT339750.28969637602836884No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC328120.27977976424555817No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA318960.27196926003828853RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC311520.26562535705771145No Hit
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA299430.2553165147142737RNA PCR Primer, Index 1 (100% over 28bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA299010.2549583911589185No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG287980.24555338445518662No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA282910.2412303215369708RNA PCR Primer, Index 1 (100% over 28bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA282170.24059934193944027No Hit
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC279280.23813511080854408No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA273940.2335818256047428RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC269530.22982152827351363No Hit
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC266410.22716118186230388RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC262720.22401481062596929RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA261740.22317918899680722RNA PCR Primer, Index 1 (100% over 23bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG250070.21322847020872462Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG248870.21220526005056706No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC245250.20911857607345832RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA242530.20679929971496777RNA PCR Primer, Index 1 (100% over 28bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG242360.2066543449425621No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT241300.2057505093028562RNA PCR Primer, Index 1 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC237160.20222043425721253No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA237060.20213516674403273RNA PCR Primer, Index 1 (100% over 23bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT233320.19894616175110824RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG232120.19792295159295065Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG229440.195637782239732No Hit
CTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTCTCGG226940.19350609441023703No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA225130.19196275242168265RNA PCR Primer, Index 1 (100% over 22bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC223430.19051320469762606No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT223280.19038530342785637No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC216860.18491112908171323No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA208350.1776548637101123RNA PCR Primer, Index 1 (100% over 22bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG206300.17590687968992638RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA205800.1754805421240274RNA PCR Primer, Index 1 (100% over 23bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG205620.17532706060030376RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC195500.16669798826650803RNA PCR Primer, Index 1 (100% over 27bp)
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA194400.16576004562153024No Hit
GCGGGTGATGCGAACTGTAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA192320.1639864813473904RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA189610.16167573174021785No Hit
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT188330.16058430757151643No Hit
AGTGGAGAAAACAGAAAATTCTGAGTTGGAATTCTCGGGTGCCAAGGAAC186600.1591091795935059RNA PCR Primer, Index 1 (100% over 24bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA185690.1583332452235697RNA PCR Primer, Index 1 (100% over 32bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC181550.15480317017792602No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG180720.15409544981853368Illumina Small RNA Adapter 2 (100% over 21bp)
CAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG178700.15237304605230173RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT175540.14967859263582006RNA PCR Primer, Index 1 (100% over 30bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC174960.14918404105937721No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA172070.14671980992848102RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC171380.1461314640875404No Hit
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC169560.14457959534766807RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC166200.1417146069048268RNA PCR Primer, Index 1 (100% over 27bp)
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCC164010.13984724836618917RNA PCR Primer, Index 1 (100% over 27bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA163130.13909689425020694No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC162490.1385511821658562RNA PCR Primer, Index 3 (100% over 50bp)
ACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGG156840.13373356767119757No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG154800.13199411040232964No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC153320.1307321512072686No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG152780.1302717066360977No Hit
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT152640.13015233211764599RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT152380.1299306365833785RNA PCR Primer, Index 1 (100% over 25bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG151080.1288221589120411No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCT149410.12739819144193845No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG147810.12603391123106167Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA146320.12476342528468265RNA PCR Primer, Index 1 (100% over 28bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT144460.12317744953953837RNA PCR Primer, Index 1 (100% over 25bp)
CTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGAT141440.12060237064150843No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA139740.11915282291745184RNA PCR Primer, Index 1 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA139220.11870943184891687No Hit
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG136650.11651805676019603No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA136210.11614287970220492No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG134680.11483828675055399No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG134510.11469333197814831No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC134050.11430110141752124RNA PCR Primer, Index 1 (100% over 27bp)
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA133840.11412203963984367No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC131500.11212677983143636RNA PCR Primer, Index 1 (100% over 24bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA131290.11194771805375878RNA PCR Primer, Index 1 (100% over 28bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT131170.11184539703794302RNA PCR Primer, Index 1 (100% over 25bp)
CACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGG130420.11120589068909453No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA129120.11009741301775712RNA PCR Primer, Index 1 (100% over 28bp)
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA126920.10822152772780153No Hit
TTTCTGATGGATTAGTGGAGAAAACAGAAAATTCTGAGTTGGAATTCTCG126370.10775255640531264No Hit
CGCGGGTGATGCGAACTTGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA124400.1060727863956706RNA PCR Primer, Index 1 (100% over 22bp)
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCATGGAATTCTCGG123890.10563792207845361No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGC123580.10537359278759624No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG122520.10446975714789038No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGGG122440.10440154313734654No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT119340.10175825022877275No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC117550.10023196174285431RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)