FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001348311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43208944
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA665174315.394366036809416No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC18512314.2843699211903905No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT17916344.146442458765018No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG15787473.653750482770419No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA12879242.9806884426520583No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC12030972.7843702914840964No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG11595862.6836712325114913No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG7813451.8082945975259195No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAG7375971.7070470410015113No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC6598561.5271282723317654No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA5701171.3194421043939422No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA4762271.1021491291247478No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA4006680.9272802408686498No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG3986920.9227071136012951No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG3519090.8144355483438799No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT3369950.779919546286528No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT3185730.7372848547282248No Hit
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA3043660.704405087983636No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCG2983290.6904334435944558No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2768500.6407238279185902No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA2675010.6190871038181355No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA2602420.6022873412504596No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAATGGAGGCAAAAAAA2520500.5833283035104955No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA2497850.5780863332369335No Hit
GTTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACA2423790.5609463633270001No Hit
TCCCAATGATGAGTTGCCATGCTAATACTGAGCCACCAGGTAGGGCAGTG2246050.5198113612774244No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG2154050.49851947319055057No Hit
CTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGAT2048900.47418423370865065No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCTGAGGACACAGTGC1989920.4605342819764353No Hit
TCGCGTGATGACATTCCCCGGAATCGCTGTACGGCCTTGATGAAAGCACA1890640.43755755752790443No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1844100.42678663935874017No Hit
TCGCGTGATGACATTCGCCGGAATCGCTGTACGGCCTTGATGAAAGCACA1782960.4126367911236155No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA1709780.3957004827519043No Hit
CTCGTACGACTCTTAGCTGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1684450.38983827052102915No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG1670850.3866907740212304No Hit
TACGGGGATGATTTTACTAACTGAACTCTCTCTTTCTGATGGATTAGTGG1649700.381795954096911No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1635000.37839388067433444No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA1615770.3739434131970455No Hit
GTCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCAC1583230.3664125649541447No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1581780.3660769862832103No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT1571500.3636978492230683No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTATTGCTGATGTGTAA1492030.3453058237202002No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1478810.3422462719755428No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA1440300.33333376534265685No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC1407150.3256617426243974No Hit
TACGGGGATGATTTTACAAACTGAACTCTCTCTTTCTGATGGATTAGTGG1405300.3252335905269983No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAAC1398820.32373390101827065No Hit
TCGCGTGATGACATTCTTCGGAATCGCTGTACGGCCTTGATGAAAGCACA1390580.3218268884330985No Hit
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG1334850.3089290957909085No Hit
GGTCAGTGATGAGCAACATTCACCATCTTTCGTTTGAGTCTCACGGCCAT1285490.29750553496516835No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA1218010.2818883979205787No Hit
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGAT1193830.2762923342907894No Hit
GTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG1191560.2757669800956024No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA1184290.2740844580696071No Hit
TCGCGTGATGACATTCTGCGGAATCGCTGTACGGCCTTGATGAAAGCACA1165520.2697404500327525No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG1137070.2631561650754529No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG1132930.2621980301115436No Hit
CGACTCTTAGCGGTGGAGCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1034800.23948745426409865No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAA1031950.2388278686005379No Hit
ATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC1020140.23609463818416854No Hit
CTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAG982220.22731867735531794No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAATGGAGGCAAAAAA980230.2268581245586562No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC974590.22555283924550434No Hit
TCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGC973860.22538389274220633No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC946200.21898244030217448No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC944150.21850800149154304RNA PCR Primer, Index 6 (100% over 50bp)
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG905010.2094496917119752No Hit
CGACTCTTAGCGGTGGAACACTCGGCTCGTGCGTCGATGAAGAACGCAGC884900.20479556269646396No Hit
CTTCTATGATGATTTTATCAAAATGACTTTCGTTCTTCTGAGTTTGCTGA878980.20342547598478689No Hit
GTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAA870070.20136340291028637No Hit
TCGCTGTGATGAGTGATTGTTAAACATTCGTAGTTTCCACCAAAAGCTTG850790.19690136375468933No Hit
GGGCGGTGATGACCCCAACATGCCATCTGAGTGTCGGTGCTGAAATCCAG845670.19571642389594157No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA843220.19514941165884545No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC808400.187090894885096No Hit
GCCGGTGATGAGAACTTCTCCCACTCACATTCGAGTTTCCCGACCATGAG790270.1828950043305849No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT775530.17948367356536182No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTA739090.17105023441443049No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG736320.17040916343616266No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG728570.1686155532984097No Hit
CGGCCGGTGATGAGAACTTCTCCCACTCACATTCGAGTTTCCCGACCATG722740.16726629560768713No Hit
CTCTGTGATGGAGCCCATGCGTGTCATCTGAGCCTCTGGCTTCCCTGCCA719770.166578937916187No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA661910.1531881917780726No Hit
GTGTATGATGACAACTCGGTAATGCTGCATACTCCCGAGTGCGCGGTGGG657410.1521467407303451No Hit
GATCGATGATGACTTCCATATATACATTCCTTGGAAAGCTGAACAAAATG644760.14921910611839995No Hit
TTCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAA630350.14588414843001024No Hit
GGTCAATGATGTAATGGCATGTATTAGCTGAATCTAAAGTTGATGTGAGT621220.14377115997095416No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC597750.13833941417313972RNA PCR Primer, Index 5 (97% over 35bp)
TGCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAA596900.1381426956419023No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGAT592880.13721233270593236No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT592760.13718456067799298No Hit
CCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAGG581580.13459713340830548No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT576010.13330804844478494No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT573680.13276880823562826No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA560410.12969768481266286No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACACCTCGTATGAAAACTGCAC559560.12950096628142543No Hit
TTCTATGATGATTTTATCAAAATGACTTTCGTTCTTCTGAGTTTGCTGAA530620.12280327887670664No Hit
TATCTGTGATGATCTTAGCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA528260.12225709566056508No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA513330.11880179251777133No Hit
CTCGTACGACTCTTAGCCGTGGATCACTCGGCTCGTGCGTCGATGAAGAA508100.11759139496674577No Hit
ACCCTCCTTTTCTTGGCGGGGATCGGGCTTGTGGTGCCGCTCCCCGTAAT506030.11711232748479111No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA491220.11368479636993674No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC489170.11321035755930532No Hit
CGCGACCTCAGATCAGATGTGGCGACCCGCTGAATTTAAGCATATTAGTC488380.11302752504203759No Hit
GTGGCCCTGACTGAAGACCAGCAGTTGTACTGTGGCTGTTGGTTTCAAGC480240.11114365581348158No Hit
GTCTGTGATGAATTGCTTTGACTTCTGACACCTCGTATGAAAACTGCACG477020.110398439730441No Hit
TTGCAGTGATGACTTGCTAATCAAATCTGTCAATCCCCTGAGTGCAATCA464850.10758189323025343No Hit
CTCGTACGACTCTTAGCAGTGGATCACTCGGCTCGTGCGTCGATGAAGAA451340.10445522575140925No Hit
CGCGACCTCAGATCAGAGGTGGCGACCCGCTGAATTTAAGCATATTAGTC446770.1033975743540504No Hit
TCCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAG432980.10020610547668093No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)