FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001348312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16580102
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT3803662.2941113390014127No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA3582362.1606380949888004No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC3510472.1172788924941477No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA3223421.9441496801406892RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT2283411.3771990063752322No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2131641.2856615719251907RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1999011.2056680954073744RNA PCR Primer, Index 1 (100% over 22bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT1596410.9628469113157446No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1296530.7819795077255857No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1210490.7300859789644237RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1150740.6940488062136168No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG998030.6019444271211359No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG936330.5647311458035663No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT866570.5226566157433772No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA815950.49212604361541323No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG811050.4891706938835479No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC730210.44041345463375314RNA PCR Primer, Index 1 (100% over 27bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT702980.4239901539809586No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC695240.4193219076698081RNA PCR Primer, Index 1 (100% over 26bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC669280.40366458541690514No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA650210.392162846766564RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC628800.3792497778361074No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT626880.3780917632472949No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC625860.3774765679969882No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC621490.37484087854224296No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG618960.3733149530684431No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA614300.3705043551601794No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC613960.3702992900767438RNA PCR Primer, Index 1 (100% over 27bp)
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG586240.35358045445076275No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG577760.3484658900168407No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC562280.3391293973945395No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA550780.33219337251363107No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA549980.3317108664349592RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA517940.3123864979841499No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA510510.3079052227784847RNA PCR Primer, Index 1 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG501690.3025855932611271Illumina Small RNA Adapter 2 (100% over 21bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT498520.30067366292438974RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC496820.2996483375072119No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT481300.2902877195809772No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA479940.289467459247235No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG477160.2877907506238502No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC465020.28046872088000424No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATTC454280.27399107677383405No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC432790.26102975723551036No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCT432230.26069200298044004No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC425760.2567897350691811No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT421560.2542565781561537No Hit
CTGGTATTGCAGTACTTCCAGGAACGGTGCACCATGGAATTCTCGGGTGC414800.2501794017913762No Hit
CTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTCTCGG413830.24959436317098654No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC386060.23284537091508845RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT380010.22919641869513224No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC379610.22895516565579632RNA PCR Primer, Index 1 (100% over 27bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA369480.2228454324346135No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC355420.214365388101955No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT351050.21172969864720978No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC345580.2084305633342907RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA342140.20635578719600156RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT339960.20504095813162065No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG328360.19804461999087822No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG325460.19629553545569262No Hit
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC319180.19250786273811826RNA PCR Primer, Index 1 (100% over 27bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT319160.19249580008615147No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG305680.18436557266053008No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCG301660.1819409796152038No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG300870.18146450486251534Illumina Small RNA Adapter 2 (100% over 21bp)
TTTCTGATGGATTAGTGGAGAAAACAGAAAATTCTGAGTTGGAATTCTCG298840.18024014568788538No Hit
AATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAAT296510.17883484673375352No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG295930.1784850298267164Illumina Small RNA Adapter 2 (100% over 21bp)
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC286260.17265273760076988No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC278320.16786386476995135No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA274820.16575290067576182RNA PCR Primer, Index 1 (100% over 23bp)
GCGGGTGATGCGAACTGTAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA262880.15855149745158384RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATATGGAATTC260640.15720048043130253No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATGGAATTCTC259570.15655512855107886No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG255370.15402197163805142No Hit
AGTGGAGAAAACAGAAAATTCTGAGTTGGAATTCTCGGGTGCCAAGGAAC255160.15389531379240007RNA PCR Primer, Index 1 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA253930.15315346069644203No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTGGAATTCT253130.15267095461777014No Hit
ACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGG240430.14501117061885385No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTTGGAATTCTC233660.14092796292809298No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA231860.1398423242510812No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA230780.13919094104487417No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGGG228830.13801483247811142No Hit
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAA227010.13691713114913287No Hit
TGCTGTGATTTCTGGCTTCAAAGTAAACGCTCTGAAGTGGAATTCTCGGG226880.1368387239113487No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA225620.13607877683744046RNA PCR Primer, Index 1 (100% over 23bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC221370.133515463294496RNA PCR Primer, Index 1 (100% over 24bp)
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAATGGAATT220880.13321992832130947No Hit
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG219340.1322911041198661No Hit
CACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGG218940.13204985108053016No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT216780.13074708466811602No Hit
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG216420.1305299569327137No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT215550.13000523157215801RNA PCR Primer, Index 1 (100% over 25bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA214190.12918497123841577RNA PCR Primer, Index 1 (100% over 28bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT212970.12844914946844116No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTGGAATTC212290.12803901930157005No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC212110.12793045543386886No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCTC211300.12744191802921356No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG210150.12674831554112273No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA207660.12524651537125644No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA206640.12463132012094981RNA PCR Primer, Index 1 (100% over 22bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG202760.12229116563939112Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC200800.12110902574664498RNA PCR Primer, Index 1 (100% over 24bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA200310.12081349077345846No Hit
CGCGGGTGATGCGAACTTGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA195330.11780989043372592RNA PCR Primer, Index 1 (100% over 22bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT194350.11721882048735285No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCATGGAATTCTCGG194110.1170740686637513No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA192430.11606080589854031RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC190840.11510182506717992No Hit
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG190510.11490279130972776No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG185220.11171221986450988No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG184820.11147096682517393No Hit
GGTCGATGATGATTGGTAAAAGGTCTGATTTGGAATTCTCGGGTGCCAAG180400.10880512074051173No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA179850.1084733978114248RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC178030.10737569648244624No Hit
CTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCTCGGG177080.10680272051402338No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA175880.10607896139601554RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG174330.10514410586858874Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC174160.10504157332687097RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC171350.10334677072553594RNA PCR Primer, Index 1 (100% over 26bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG171040.10315979962005059No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG167620.10109708613372824No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATTGGAATTCTCGGGTG167350.10093424033217648No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC166220.10025270049605244RNA PCR Primer, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)