FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001348313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42982859
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA736677417.13886458785815No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC15612733.6323153841395244No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT15376483.5773516135815906No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG15306593.561091643531669No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG14151333.2923193871305765No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC11713482.725151437692872No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA11547502.686536044519514No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG7825621.820637384777034No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAG7812241.8175245159936892No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA7522481.7501115968111844No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC7030661.6356892406808026No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA4523321.0523543815454435No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCG4357281.0137250293192457No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG4268570.993086569695143No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT4128070.9603991209612185No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA4077420.948615353855359No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG3885030.903855650923546No Hit
GTTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACA3313820.7709631413768916No Hit
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA3241390.7541122380900721No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA2869160.667512600778836No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT2864250.6663702849547537No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA2596590.6040989502350227No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAATGGAGGCAAAAAAA2518690.5859754466309465No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2493020.5800032985241861No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA2298540.534757355251776No Hit
GTCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCAC2283940.531360652394016No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG2240240.5211938089088024No Hit
TCGCGTGATGACATTCCCCGGAATCGCTGTACGGCCTTGATGAAAGCACA2142240.4983940226032894No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT2105160.4897673279481014No Hit
TCCCAATGATGAGTTGCCATGCTAATACTGAGCCACCAGGTAGGGCAGTG2083100.48463504952055425No Hit
TCGCGTGATGACATTCGCCGGAATCGCTGTACGGCCTTGATGAAAGCACA1954740.4547719824779454No Hit
CTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGAT1890720.439877673097548No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1787000.41574712375461115No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA1777650.4135718380203606No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA1685310.39208885569943125No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT1645970.38293637005393244No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG1578220.36717427288864146No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAA1548590.3602808272944338No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG1525870.3549949992856455No Hit
TACGGGGATGATTTTACTAACTGAACTCTCTCTTTCTGATGGATTAGTGG1518560.35329432134795874No Hit
CTCGTACGACTCTTAGCTGTGGATCACTCGGCTCGTGCGTCGATGAAGAA1517140.35296395709740946No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC1497660.348431917941987No Hit
GTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG1472250.34252025906420047No Hit
TCGCGTGATGACATTCTTCGGAATCGCTGTACGGCCTTGATGAAAGCACA1459660.33959118447658404No Hit
GGTCAGTGATGAGCAACATTCACCATCTTTCGTTTGAGTCTCACGGCCAT1378450.32069760645749507No Hit
TCGCTGTGATGAGTGATTGTTAAACATTCGTAGTTTCCACCAAAAGCTTG1346810.3133365325931437No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1268870.29520372295384073RNA PCR Primer, Index 5 (100% over 50bp)
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCTGAGGACACAGTGC1254950.2919652226949352No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTATTGCTGATGTGTAA1234980.2873191846079853No Hit
TCGCGTGATGACATTCTGCGGAATCGCTGTACGGCCTTGATGAAAGCACA1212260.28203335659919687No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG1206850.2807747153347803No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA1204130.280141904939362No Hit
GGGCGGTGATGACCCCAACATGCCATCTGAGTGTCGGTGCTGAAATCCAG1184440.27556100909899917No Hit
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGAT1173930.27311584834317326No Hit
TACGGGGATGATTTTACAAACTGAACTCTCTCTTTCTGATGGATTAGTGG1172150.2727017297755833No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA1143180.26596183376261684No Hit
GAGGAATGATGACAAGAAAAGGCCGAATTGCAGTGTCTCCATCAGCAGTT1092910.25426647399141133No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAAC1076330.2504091223899276No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAATGGAGGCAAAAAA1074250.2499252085581371No Hit
ATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC1055980.24567467696832357No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA1021650.23768777223497395No Hit
CGACTCTTAGCGGTGGAGCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1008250.23457025043401603No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT968310.22527817426011612No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA962600.22394973773149895No Hit
CTTCTATGATGATTTTATCAAAATGACTTTCGTTCTTCTGAGTTTGCTGA957490.22276089173128294No Hit
AGGAATGATGACAAGAAAAGGCCGAATTGCAGTGTCTCCATCAGCAGTTT957250.2227050555199225No Hit
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGAT930630.216511889076527No Hit
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG898190.20896469450764082No Hit
TGCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAA895140.20825510932160188No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC888950.20681500037026387No Hit
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG885320.2059704776734372No Hit
CGACTCTTAGCGGTGGAACACTCGGCTCGTGCGTCGATGAAGAACGCAGC880160.20476999912918775No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTTGTATAA872800.2030576886474676No Hit
CGGCCGGTGATGAGAACTTCTCCCACTCACATTCGAGTTTCCCGACCATG846630.19696921510037294No Hit
CTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAG825390.19202771039497396No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG768890.17888293563720364No Hit
GCCGGTGATGAGAACTTCTCCCACTCACATTCGAGTTTCCCGACCATGAG757710.17628189879132983No Hit
GATCGATGATGACTTCCATATATACATTCCTTGGAAAGCTGAACAAAATG733860.17073317528738607No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG728160.1694070652675756No Hit
TATCTGTGATGATCTTAGCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA726870.16910694563151324No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC722510.1680925877917986No Hit
TCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGC716010.16658035706745333No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTA712730.16581726217886067No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT711340.16549387745473143No Hit
GTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAA705420.1641165842411739No Hit
TTCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAA628020.14610940607743195No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC615840.1432757183508896No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG601120.13985109738744927No Hit
CTCTGTGATGGAGCCCATGCGTGTCATCTGAGCCTCTGGCTTCCCTGCCA600850.13978828164966875No Hit
TTCTATGATGATTTTATCAAAATGACTTTCGTTCTTCTGAGTTTGCTGAA597160.13892979990000198No Hit
GGTCAATGATGTAATGGCATGTATTAGCTGAATCTAAAGTTGATGTGAGT556080.12937250172214No Hit
CCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAGG547980.12748802958872513No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA506170.11776089626797509No Hit
GTGTATGATGACAACTCGGTAATGCTGCATACTCCCGAGTGCGCGGTGGG503990.11725371734811778No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA482930.11235408980123913No Hit
TTGCAGTGATGACTTGCTAATCAAATCTGTCAATCCCCTGAGTGCAATCA469720.10928077166760825No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT466450.10852000328782224No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGC461060.10726601504101901No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA456380.10617720891949044No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAC455290.10592361945956177No Hit
CTCGTACGACTCTTAGCCGTGGATCACTCGGCTCGTGCGTCGATGAAGAA453120.10541876704851112No Hit
TCCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAG450860.1048929760582003No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACACCTCGTATGAAAACTGCAC447050.10400657620285333No Hit
CCTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGC445310.10360176367049015No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT438050.10191271827683683No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA437360.10175218916917556No Hit
CGCGACCTCAGATCAGATGTGGCGACCCGCTGAATTTAAGCATATTAGTC434650.10112170528256391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)