FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001348361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4329470
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT2530905.845750172653927No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG1250812.889060323780971No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC1193882.757566168607243No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC778761.7987421093113014No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA749341.7307892190037117RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT668141.5432373939535324No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA652631.5074131475677162RNA PCR Primer, Index 1 (100% over 22bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA592141.3676962769114926No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT510251.1785507232986947No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC467131.0789542368927374RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT454381.0495049047573952No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA399350.9223992775097183No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG388540.8974308633620282No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG353270.8159659265452816No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA341840.7895654664427747No Hit
AATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAAT328440.7586147958064151No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA310250.7166004152933269No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT307450.7101331109812518No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATTC254750.5884092048218373No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC248040.5729107719882572No Hit
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC247500.5716635061566427No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT224440.5184006356436238No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCT219640.5073138282514951No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC179310.4141615486422125RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA175800.4060543207367183RNA PCR Primer, Index 1 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT174680.4034673990118883No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA169380.3912257158497461No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG158780.36674234952546153No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC158350.36574915636325No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT157810.3645018905316355No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA154360.35653324771854294RNA PCR Primer, Index 1 (100% over 28bp)
CTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTCTCGG146750.3389560384989386No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC141390.3265757702443948No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC134430.31049989952580803RNA PCR Primer, Index 1 (100% over 26bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC131490.3037092299981291No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGGG126240.2915830344129882No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAATGGAATT124560.28770265182574306No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT119080.27504521338639604No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG116030.2680004711893142No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT113680.2625725550702511No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC112500.2598470482530194No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATTGGAATTCTCGGGTG110960.2562900308813781No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC105210.24300895952622378RNA PCR Primer, Index 1 (100% over 27bp)
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTTGGAATTCTC103930.2400524775549894No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA103180.23832016389996927No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT102850.2375579458917604No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCATGGAATTC100440.23199144468029573No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG99730.23035152108687668No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGG97610.22545484782201977No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC89420.20653798270919999No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATGGAATTCTC88720.20492115663118116No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG88450.2042975237153739Illumina Small RNA Adapter 2 (100% over 21bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC86550.19990899578932295No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA86420.19960872808911945No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCTC83550.19297974116924244No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCG80440.18579641387975895No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG78370.18101522819190338No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC76810.17741201578946153RNA PCR Primer, Index 1 (100% over 26bp)
TTTCTGATGGATTAGTGGAGAAAACAGAAAATTCTGAGTTGGAATTCTCG76560.1768345779044548No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA75780.17503297170323387RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA75480.17434004624122582RNA PCR Primer, Index 1 (100% over 28bp)
ATCGCTGTACGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGAT75090.17343924314061537No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG74870.17293109780180946No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCATGGAATTCTCGG74580.17226126985520168No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGC72360.16713362143634208No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA70780.16348421400309968No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATATGGAATTC69950.1615671202248774No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA69750.16110516991687204No Hit
CCATCTGAGTGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATT68540.15831037055343958No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGGAATTCTCGGGTGC68320.15780222521463366No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC67970.15699381217562428No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTGGAATTC67810.15662425192921997No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA65140.1504572153173483No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG63530.1467385153379051No Hit
TGCTGTGATTTCTGGCTTCAAAGTAAACGCTCTGAAGTGGAATTCTCGGG63460.14657683273010322No Hit
ACCAGTGATGAGTTGAATACCGCCCCAGTCTGATTGGAATTCTCGGGTGC63410.14646134515310189No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT62690.14479832404428256RNA PCR Primer, Index 1 (100% over 25bp)
CTCTACTGAACTGCCATGAGGAAACTGCCATGTCACCCTTCTGACTTGGA62530.14442876379787828No Hit
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG61000.1408948439416372No Hit
CACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGG60130.13888536010181385No Hit
ACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGG60040.13867748246321143No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC59980.13853889737080982No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTGGAATTCT59340.13706065638519263No Hit
TCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGTGGAATTCTCGG58670.13551312285337466No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC58260.13456612472196366No Hit
GTGGGGGTCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA57770.13343434646735053No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC56660.1308705222579207No Hit
CTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCTCGGG53770.12419534030724314No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA53000.12241683162142249RNA PCR Primer, Index 1 (100% over 22bp)
GCGGGTGATGCGAACTGAAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA52690.12170080864401416RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC52320.12084620057420423No Hit
GGTCAATGATGTAATGGCATGTATTAGCTGAATTGGAATTCTCGGGTGCC51870.11980681238119215No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT50390.11638838010195243No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG49970.11541828445514116No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC49550.11444818880832988No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA49370.11403243353112506No Hit
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG48220.11137621926009418No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC47120.10883549256606466RNA PCR Primer, Index 1 (100% over 27bp)
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT46870.10825805468105795No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG46850.10821185965025742No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC46640.1077268118268518RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC46490.10738034909584776No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA46110.10650264351063755No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG46090.10645644847983704No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGTGGA45760.10569423047162817No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG45750.1056711329562279No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGTGGAATTCTCGGGTGC43930.10146738515337905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)