FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001348476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12899557
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA5655214.384034273425049No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC4125913.1984896845682376RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC3745382.9034950580085814RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC2658272.0607451868308346RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT2359511.8291403340440295No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA2120771.644064210887242RNA PCR Primer, Index 1 (100% over 23bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC1801071.3962262424980951No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT1553181.204056852495012No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1544581.1973899568799147RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA1351291.0475476018284968RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC1345571.0431133410240367RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1309221.0149340787439445No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1101610.8539905672729692RNA PCR Primer, Index 1 (100% over 27bp)
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG1077090.8349821625657377No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA977850.7580492880491942RNA PCR Primer, Index 1 (100% over 28bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT968180.7505529065843114No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC861690.6679996840201566RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT842510.6531309563576486RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC717620.5563136780588667RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA696200.5397084566547518RNA PCR Primer, Index 1 (100% over 28bp)
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC622650.4826909947372612No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG578040.4481084117849939No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC566290.4389995718457619RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC564730.43779022798999995RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA540250.418812832099583RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA533760.4137816515714455No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA527980.4093008775417637No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC513550.39811444687596637No Hit
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC474170.3675862667221828RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT470100.3644311196113169No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC460530.35701226018847004RNA PCR Primer, Index 2 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA442180.34278696547486087RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA439950.3410582239374577RNA PCR Primer, Index 1 (100% over 28bp)
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG433310.33591076034626616No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC430410.3336626211272216No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC427460.33137572088715916No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA426100.3303214211154693No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT406930.3154604456571648RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT390620.3028166006010904No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC373720.2897153754970035No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC362490.28100965017635876No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG355970.27595521303561044No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT347400.2693115740331238No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT346710.26877667194307525RNA PCR Primer, Index 1 (100% over 25bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCT345700.26799369931851147No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA341210.2645129596310943RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC332140.25748171041842755No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC319390.24759765005883538RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC317230.2459231739508574RNA PCR Primer, Index 1 (100% over 27bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA314690.24395411408314255No Hit
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC305410.23676006858220014RNA PCR Primer, Index 1 (100% over 26bp)
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA301490.23372120453438827No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATTC289980.2247984174960427No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA289930.22475965647502466No Hit
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA289640.22453484255312023RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA289460.22439530287745538RNA PCR Primer, Index 1 (100% over 22bp)
CTGGTATTGCAGTACTTCCAGGAACGGTGCACCATGGAATTCTCGGGTGC288480.22363558686550244No Hit
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG288210.22342627735200518RNA PCR Primer, Index 1 (100% over 29bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC287730.22305417155023233No Hit
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT270690.20984441558729497No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG259520.20118520349187186RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG254730.19747189767834664No Hit
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC250600.19427023734225912RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT250500.1941927153002231No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA249590.19348726471769537No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG248180.1923942039249875No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC244740.1897274456789485RNA PCR Primer, Index 1 (100% over 27bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT243650.18888245542075593No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC242490.18798319973313812RNA PCR Primer, Index 1 (100% over 27bp)
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG239790.18589010459816566No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT232880.18053333149347686RNA PCR Primer, Index 1 (100% over 30bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG231140.17918444796205016No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG226610.1756726994578186Illumina Small RNA Adapter 2 (100% over 21bp)
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG220550.17097486371043594No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA218510.1693934140529012RNA PCR Primer, Index 1 (100% over 22bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA217040.16825384003497174RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT213630.16561033840154354No Hit
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG209810.1626489963957677Illumina Small RNA Adapter 2 (100% over 21bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT204270.15835427526697235No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT204140.15825349661232552RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC203230.15754804602979777No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA200190.15519137595190285RNA PCR Primer, Index 1 (100% over 23bp)
ACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGG196130.15204398104524056No Hit
GGTCAATGATGTAATGGCATGTATTAGCTGAATTGGAATTCTCGGGTGCC189340.14678023439099497No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTGGAATTCT188450.14609028821687442No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC186350.14446232533411807RNA PCR Primer, Index 1 (100% over 31bp)
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCTC182050.1411288775265693No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCG179260.13896601255376442No Hit
ATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGTGC171250.13275649698667946No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA169790.13162467517295362RNA PCR Primer, Index 1 (100% over 32bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA168990.13100449883666548RNA PCR Primer, Index 1 (100% over 23bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG164630.12762453780389513RNA PCR Primer, Index 1 (100% over 29bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG163270.1265702380322053No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG159730.12382595774413029No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC158420.12281041899345846No Hit
CACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGG158230.12266312711359004No Hit
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA158110.1225701006631468No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA157340.12197318093946946RNA PCR Primer, Index 1 (100% over 32bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG157190.12185689787641546Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC156670.12145378325782816No Hit
TGCTGTGATTTCTGGCTTCAAAGTAAACGCTCTGAAGTGGAATTCTCGGG155830.12080259810472561No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC154190.11953123661533492No Hit
CTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGT153790.11922114844719087No Hit
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG153470.11897307791267561No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA153250.11880252942019637RNA PCR Primer, Index 1 (100% over 28bp)
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTGGAATTC152810.1184614324352379No Hit
AATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAAT150810.11691099159451754No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC147500.11434501200312538No Hit
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT142990.11084876790730101RNA PCR Primer, Index 1 (100% over 25bp)
GACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGTGCCA142060.11012781291636604No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA139880.10843783239998088RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG139330.10801146116878277No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC136750.10601139248425354RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC135440.10499585373358171RNA PCR Primer, Index 1 (100% over 27bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATATGGAATTC134330.10413535906698192No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCC133000.10310431590790288RNA PCR Primer, Index 1 (100% over 27bp)
CGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGTGCCAAGG132430.10266244026829757Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGGTGCCAA130770.1013755743704997No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT130140.10088718550567279No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)