FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001348478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13315777
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT5805494.359858234333603No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA3837772.882122462699698RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT3331132.501641473869681No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC2437481.8305202918312617No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC2171031.630419313871057RNA PCR Primer, Index 1 (100% over 26bp)
CTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGT2017441.5150749370464824No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1960691.4724563200480152RNA PCR Primer, Index 1 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT1949071.463729829659959No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGG1861271.3977930089997752Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGA1853361.3918526872295924RNA PCR Primer, Index 1 (100% over 22bp)
TCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGG1491631.1201974920427098No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGG1417701.0646768866736054Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1267720.9520435795823255No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1203240.9036198188059172No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG1192040.8952087437330919Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1110280.8338078957014674No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1102480.8279501827043213RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1096110.823166383756652No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA1024950.7697260174903799RNA PCR Primer, Index 1 (100% over 23bp)
TGGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGG1018410.7648145504389267Illumina Small RNA Adapter 2 (100% over 21bp)
TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTG1003750.753805053959675No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT977770.7342943637461036No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC898250.6745757307290442No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC881500.6619966675620957No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTGGAATTCT851110.6391741165385992No Hit
AATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGG805020.6045610406362317No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA798010.5992966088272581No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA790890.5939495682452477RNA PCR Primer, Index 1 (100% over 28bp)
GCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA784790.5893685362859411RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG767880.5766693149036666No Hit
TCACAAAGATGAGTGGTGAAAATCTGATCTGGAATTCTCGGGTGCCAAGG767870.5766618050152087Illumina Small RNA Adapter 2 (100% over 21bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGC689460.5177767696169738No Hit
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTCG682940.5128803223424363No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT675230.5070901983414111No Hit
TGGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAG669170.502539205935936No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA662860.4978004663190139No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC653710.490928918380054No Hit
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG648800.48724156314723505RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC636500.478004400344043No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC632170.4747526186417811No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA608020.4566162380160016RNA PCR Primer, Index 1 (100% over 22bp)
AGTAATGATGAATGCCAACCGCTCTGATGTGGAATTCTCGGGTGCCAAGG542320.407276270847732Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC536730.4030782431997772No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGG530030.39804661793299784Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA526740.39557586463035543RNA PCR Primer, Index 1 (100% over 22bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT512400.38480668458175593RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC508830.38212565440229285RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCT503960.37846833872330543No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC497650.37372959910638337RNA PCR Primer, Index 1 (100% over 26bp)
TAATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCG497290.3734592431218997No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA487300.3659568645524779RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA427360.3209425931359469RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC426910.32060464815534234RNA PCR Primer, Index 1 (100% over 27bp)
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTGGAATTCT425440.319500694552034No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGGAATTCTCGGGTGCC416400.31271175538611073No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGGAATTCTCGG408130.3065010776314443No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT404830.30402281444034396No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA403840.30327933548301383RNA PCR Primer, Index 1 (100% over 28bp)
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGTGGAATTCTCGGG397420.2984579870930551No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT385700.2896563978204201RNA PCR Primer, Index 1 (100% over 25bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGGA374260.28106508542460573RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG371880.27927773197163036No Hit
ATTAATGATGAGATATAACCTTGACTGAAGCTGATGATGAGTTGGAATTC370810.2784741739066372No Hit
CGGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCTGGAATTCTC368130.27646152379992545No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG358600.26930460009956614Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA344680.2588508353661975RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC344400.25864055848937695RNA PCR Primer, Index 1 (100% over 26bp)
CTCTCACTGATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGG330240.24800655643301928No Hit
ACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTGGAATT327870.24622671286850176No Hit
TTCACTGATGAGAGCATTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA325520.24446188908090002RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA318130.23891208151052695RNA PCR Primer, Index 1 (100% over 28bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC302990.22754211038529706No Hit
CGCGGCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT300320.2255369701670432No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC297870.2236970474948627RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC291160.2186579123396254No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCT290600.21823735858598411No Hit
TTACTTGATGACAATAAAATATCTGATTGGAATTCTCGGGTGCCAAGGAA283910.21321324320766258RNA PCR Primer, Index 1 (100% over 23bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA280950.21099031622413023RNA PCR Primer, Index 1 (100% over 28bp)
AATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAAT279110.20960849674788035No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG273140.20512509333852616No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT262800.19735986867307856No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA256870.1929065048175559No Hit
TCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGTGGAATTCTCGG254580.19118674036070143No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA245680.1845029396331885No Hit
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCATGGAATTC244360.1835116343567484No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC233530.17537842515686466RNA PCR Primer, Index 1 (100% over 27bp)
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGT232980.1749653812916813No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATTC223870.1681238729065529No Hit
TTTAAGTGAATGAAAAATTACAAGATCCAACTCTGATTTGGAATTCTCGG223690.16798869491431104No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGAATTCTCGGGTGCC221110.16605114369217808No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA216590.16265667410921644No Hit
TCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCTGGAATTCTC210690.1582258399190674No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC207010.1554622009665677No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA206390.15499658788217915RNA PCR Primer, Index 1 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG201300.1511740546571184No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA201210.15110646566099747RNA PCR Primer, Index 1 (100% over 28bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTC198550.14910883533120148No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC197290.14816258938550864RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTGGAAT192510.14457286270264214No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG192270.14439262537965303No Hit
CTACCTGATGCATGATCTCTACAGTTCTGAGATGGAATTCTCGGGTGCCA187430.14075783936603925No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA185910.13961633632044154No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC185310.13916574301296875RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC184210.13833965528260198RNA PCR Primer, Index 1 (100% over 27bp)
CTCAGTGATGTAATTCCAATAGATCCTTCTGACCTGGAATTCTCGGGTGC180550.135591036107018No Hit
GCTCCGTGAGGATAAGTAACTCTGAGGTGGAATTCTCGGGTGCCAAGGAA179550.13484004726123006RNA PCR Primer, Index 1 (100% over 23bp)
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCTCGGGTGCCAAGGA177970.13365348488488504RNA PCR Primer, Index 1 (100% over 22bp)
GGTCAATGATGTAATGGCATGTATTAGCTGAATTGGAATTCTCGGGTGCC174930.13137047879368963No Hit
AGTCAATGATGGTTTTATTCATATCGTCTGAACTGGAATTCTCGGGTGCC169900.12759300489937614No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTTGGAATTCTC156720.11769497191189068No Hit
CACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTTTGGA155200.11655346886629296No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTGGAATTCTCGGGTGCCAA151850.11403765623290328No Hit
AATTCTTGAAGAAAATTTTTGTGTGTCTGATCTGGAATTCTCGGGTGCCA151410.11370722114075656No Hit
GACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTTGGAATTCTCG151380.11368469147538293No Hit
ACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTTTGGAA150710.11318152894870498No Hit
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG147260.11059061743073648Illumina Small RNA Adapter 2 (100% over 21bp)
ACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGGG146810.11025267245013189No Hit
AAACCTGATGTTACATTGTAGTGTGCTGATGTGGAATTCTCGGGTGCCAA142860.10728626650926942No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC141990.10663290621343388RNA PCR Primer, Index 11 (100% over 50bp)
TGCTGTGATTTCTGGCTTCAAAGTAAACGCTCTGAAGTGGAATTCTCGGG139180.10452262755676969No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA137250.1030732190843989RNA PCR Primer, Index 1 (100% over 28bp)
GTCCATGATGATTTCAAGTTATCCCTGTCTGAAGTGGAATTCTCGGGTGC137090.10295306086907283No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATTGGAATT136960.1028554323191204No Hit
CACTATGACCGACAGTGAAAATACATGAACACCTGAGATGGAATTCTCGG134990.10137598429291811No Hit
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATATGGAATTC134180.10076768332782984No Hit
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC133480.10024199113577825RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)