FastQCFastQC Report
Sun 14 May 2023
EGAF00001348510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26401947
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG6702742.5387294353708083TruSeq Adapter, Index 11 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC5557182.1048371924994775TruSeq Adapter, Index 11 (100% over 50bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC1485380.5626024474634389TruSeq Adapter, Index 11 (100% over 42bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT306860.1162262767969347TruSeq Adapter, Index 11 (100% over 37bp)
CGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGT294500.11154480387374462TruSeq Adapter, Index 11 (100% over 46bp)
CGGTGGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC273340.10353024343242566TruSeq Adapter, Index 11 (100% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)