FastQCFastQC Report
Sun 14 May 2023
EGAF00001348511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26401947
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6767262.563167027037817Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1893810.7172993718985953Illumina Single End PCR Primer 1 (100% over 50bp)
ACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1443960.5469142105315188Illumina Single End PCR Primer 1 (100% over 43bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG357040.13523245084917412Illumina Single End PCR Primer 1 (98% over 50bp)
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC289940.10981765852344147Illumina Single End PCR Primer 1 (100% over 47bp)
CGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC267680.1013864621423564Illumina Single End PCR Primer 1 (100% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)