FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001348514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16685691
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC6839424.098973186067032RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA4591392.7516930524483523RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC4291442.571928246783427RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC3885272.3285041057035034RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC3822902.2911247727169344No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3711922.224612693594769RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC3635812.1789987600753244RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3433242.0575953372263696RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA3020621.8103056085600528RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2550961.5288309006801095RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC2391921.4335156991700253RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC2317631.3889925205974387RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2310561.3847553571500275RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2075191.2436943726214276RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1681511.0077556871932962RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT1599130.9583840429503339No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1536910.9210946073494949RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC1364960.8180422375075747RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1216480.7290558119528883RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1050130.6293596111782245RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1043000.6250864887765212No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA1016700.6093244804785131No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA824380.4940640456544473RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA799340.47905717539657183RNA PCR Primer, Index 7 (100% over 35bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC783460.46954003882728024RNA PCR Primer, Index 1 (100% over 27bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG748540.44861192742931655No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG733480.43958622990201607No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC718500.43060847764710497RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC685780.41099886123984913RNA PCR Primer, Index 1 (100% over 33bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA675920.40508960641785824RNA PCR Primer, Index 1 (100% over 28bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT672970.40332162449849995No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT610680.3659902367843202No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA574010.34401332255283884RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA540650.32402014396646805RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC538250.3225817857947867RNA PCR Primer, Index 1 (100% over 26bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA522310.31302869027120306RNA PCR Primer, Index 1 (100% over 32bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC506530.30357148529239814RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC497390.29809373792191163RNA PCR Primer, Index 1 (100% over 26bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA474740.2845192326766689RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGTGGAATTCTCGGGTGCCAAGGAACTC463240.2776270997706957RNA PCR Primer, Index 1 (100% over 26bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA462240.2770277838658285RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC461140.2763685363704746RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT461130.2763625432114259RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC437930.26245841421850613No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC428260.25666302941844005RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG426120.2553804933820241No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA422870.25343271669120565RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTTTTGGAATTCTCGGGTGCCAAGGAACTC422170.25301319555779855RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC418960.2510893915031748No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT411900.24685822121481216No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC407190.24403544330288748RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCCTGAGTGCAATCACTGATGTCTCCATGTCTCTGAGCAATGGAATT398310.2387135180676665No Hit
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCC394300.2363102612891489RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA392450.2352015268651445RNA PCR Primer, Index 1 (100% over 28bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG360080.21580167102459227RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATTGGAATTCTCGGGTGCCAAGGAACT356400.21359618849468084RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTC352010.21096519167231373RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGCGGAAACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC350400.21000029306547746RNA PCR Primer, Index 1 (100% over 33bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC326010.19538297814576575RNA PCR Primer, Index 1 (100% over 33bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC325290.19495147069426133RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC311110.186453171163244RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC295960.17737353520450547RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC293620.17597113598711614RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA293480.17588723176043475RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACT291580.174748531541187RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG288880.17313037859804548RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA285180.1709129097500367RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC271090.16246854865045746No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC264000.1582193988849488No Hit
TGATGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA263440.15788378197822311RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC257100.1540841191413649RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCC252600.15138719756946237RNA PCR Primer, Index 1 (100% over 27bp)
TGTCAGTTTGTCAAATACCCCATGGAATTCTCGGGTGCCAAGGAACTCCA239930.14379386505479455RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG235230.14097708030191858No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA234570.1405815318047062RNA PCR Primer, Index 1 (100% over 23bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG230300.13802245289092313RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC229860.13775875389278153RNA PCR Primer, Index 2 (100% over 34bp)
CGCTACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC229300.1374231369860559No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG229120.1373152601231798RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC214520.12856524791211824RNA PCR Primer, Index 7 (100% over 50bp)
TGATGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA213750.12810377466537046RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC213590.1280078841205917RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC206670.12386061805891048RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA205600.12321935004070254RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG202780.12152927918897695RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC201420.12071420955835752RNA PCR Primer, Index 1 (100% over 26bp)
TCGTTTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA200610.12022876367541505RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC199480.11955153670291509RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTATTTGGAATTCTCGGGTGCCAAGGAACT196900.11800530166835763RNA PCR Primer, Index 1 (100% over 25bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTCTCGGGTGCCAAGG190900.11440940623915424Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA188340.11287515752269414RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTATGGAATTCTCGGGTGCCAAGGAACTC187640.11245563638928707RNA PCR Primer, Index 1 (100% over 26bp)
TGTCAGTTTGTCAAATACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAG185990.11146676514625616RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC185670.11127498405669864RNA PCR Primer, Index 1 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA184200.11039398967654382RNA PCR Primer, Index 1 (100% over 22bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC181090.10853011721240673RNA PCR Primer, Index 2 (100% over 34bp)
TGTCAGTTTGTCAAATACCCCAATGGAATTCTCGGGTGCCAAGGAACTCC177060.10611487411579179RNA PCR Primer, Index 1 (100% over 27bp)
AACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA175280.10504809180512811RNA PCR Primer, Index 1 (100% over 28bp)
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG174800.10476042017079186No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA172530.10339997306674324RNA PCR Primer, Index 1 (100% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA171330.10268079398090256RNA PCR Primer, Index 1 (100% over 28bp)
TTCACCACCTTCTCCACCCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA169210.10141024426258403RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTCTCGGGTGCCAAGG167920.1006371267453053Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATTTGGAATTC167240.10022959192999559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)