FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001348518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20041952
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC693743034.61454253557738RNA PCR Primer, Index 4 (100% over 50bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT5456052.7223146727424554No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC5321912.655385064289147No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA4543292.2668899715955813No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC3746921.8695384561344124No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA2383431.1892204910978732No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC2073911.0347844361666967No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC1934100.9650257619617091No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAG1868840.9324640633806527No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC1656250.8263915610615173RNA PCR Primer, Index 4 (100% over 38bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT1572370.784539350258897RNA PCR Primer, Index 4 (100% over 41bp)
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG1503440.7501464927168771No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG1257760.6275636225453488No Hit
CCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT1184190.5908556212488684RNA PCR Primer, Index 4 (100% over 41bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG1085400.5415640153214617No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA1062480.5301280034998587RNA PCR Primer, Index 4 (100% over 39bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA1047390.5225987967639081No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA1033740.5157880829172727RNA PCR Primer, Index 4 (100% over 40bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA944500.47126148191553396No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCTGAGGACACAGTGC921170.45962089920183424No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA917750.4579144785897102RNA PCR Primer, Index 1 (100% over 28bp)
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT904210.4511586496165643No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA870620.43439880506649253RNA PCR Primer, Index 4 (100% over 40bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCG814630.4064624044604038No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA745800.3721194422579198No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG720150.35932128766698973No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG685430.3419976257801635No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA679630.33910369608708774RNA PCR Primer, Index 2 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC646750.32269810844772007No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA644820.3217351283946793RNA PCR Primer, Index 4 (100% over 39bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT639020.31884119870160355No Hit
CCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT634980.3168254269843576RNA PCR Primer, Index 4 (100% over 41bp)
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGAT615730.30722057412371806No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG575700.28724746970754145No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT544690.27177492491749305No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG534250.26656585146995665No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA460250.2296433002134722No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG447610.2233365292961484No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG445730.22239849691287555No Hit
CGCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA435870.2174788164346467RNA PCR Primer, Index 4 (100% over 40bp)
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC433060.21607675739369098RNA PCR Primer, Index 4 (100% over 38bp)
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG432850.21597197718066585No Hit
CTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAG419150.20913631566426266No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCA403570.20136262176458658No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA395930.19755061782405228RNA PCR Primer, Index 1 (100% over 32bp)
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC392060.1956196681840172No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC386650.19292033031513098No Hit
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCC385060.19212699441651193No Hit
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC375380.18729712554944747RNA PCR Primer, Index 4 (100% over 37bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA349050.17415968264967405RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG345430.17235347135847845No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC342670.17097635998729066RNA PCR Primer, Index 4 (98% over 50bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC318270.15880189714055798No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA313240.15629216156190776No Hit
TCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGC311570.15545890939166004No Hit
CGCGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA308160.15375747831348963RNA PCR Primer, Index 4 (100% over 39bp)
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG305150.15225562859346237No Hit
CCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA297050.1482141060910634RNA PCR Primer, Index 4 (100% over 40bp)
CCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC295560.14747066553198013RNA PCR Primer, Index 4 (100% over 42bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG292240.1458141402593919RNA PCR Primer, Index 4 (100% over 45bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA287650.14352394417469916RNA PCR Primer, Index 1 (100% over 28bp)
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTA270860.13514651666664004No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG270810.13512156899687217No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCT267990.13371452042196288No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA260730.13009211877166457No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTTTAAGCATATTAGTC242760.12112592625708316No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT242720.12110596812126882No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT240210.1198535950989205RNA PCR Primer, Index 1 (100% over 30bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC234860.11718419443375575RNA PCR Primer, Index 4 (100% over 44bp)
GTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG230350.11493391462069165No Hit
ATTCGTGATGACTGATCATTTCTTCACTTTGACCAGATGTCTACTGAAGA229220.11437009728393721No Hit
CGGCCGGGCGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA226480.11300296498065658RNA PCR Primer, Index 2 (97% over 36bp)
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAA223290.11141130364946487No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT221810.1106728526243352RNA PCR Primer, Index 4 (100% over 43bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC215090.10731988580753012RNA PCR Primer, Index 1 (100% over 26bp)
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGAT213260.10640680109402517No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA212740.10614734532843907No Hit
GCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA210820.1051893548093519RNA PCR Primer, Index 4 (100% over 40bp)
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT209190.10437606077491852RNA PCR Primer, Index 4 (100% over 41bp)
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCA208640.10410163640747168No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCCAGGAACTCCAGTCACTGACC207850.10370746322513895RNA PCR Primer, Index 4 (97% over 38bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA202960.10126758112183883RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)