FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001348521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5992075
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT153348525.59188594935811TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA77282712.897485428670368TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAATAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT177180.2956905579452861TruSeq Adapter, Index 15 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT166680.27816741279106155TruSeq Adapter, Index 15 (97% over 40bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT159310.265867833763763TruSeq Adapter, Index 15 (97% over 40bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT146970.24527396603013146TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTACAGTCACATGTCAGAATCTCGTAT140210.233992398292745TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT131850.22004063700804813TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCAAGTCACATGTCAGAATCTCGTAT130090.2171034241060067TruSeq Adapter, Index 15 (97% over 40bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA117190.19557498863081654TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTTAACTCCAGTCACATGTCAGAATCTCGTAT116610.19460704346991653TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGAATCTCGTAT101200.16888974186738318TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTAACATGTCAGAATCTCGTAT98270.163999949933871TruSeq Adapter, Index 15 (97% over 40bp)
GATCGTAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT87780.14649349348931714TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAATAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA85150.14210436284592565TruSeq Adapter, Index 15 (97% over 40bp)
AGATCTGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA75040.12523207736885802TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGATCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT73300.12232824188615797TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA71290.11897381124234926TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTACAGTCACATGTCAGAATCTCGTA69710.11633699511438024TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTATGAACTCCAGTCACATGTCAGAATCTCGTAT68200.11381699995410605TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCAAGTCACATGTCAGAATCTCGTA66550.11106336285844219TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCAACAATCTCGTA65980.11011210640721285TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGAACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT63880.10660747737636796TruSeq Adapter, Index 15 (97% over 40bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA62920.10500536124798171TruSeq Adapter, Index 15 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)