FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001348522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5992075
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG118355019.75192233074519Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC84694914.134485966881256Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG313170.5226403207569998Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG215370.3594247401776513Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGCGTAGATCTCGGTGGTCGCCG108800.1815731612171076Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAATAGTGTAGATCTCGGTGGTCGCCG108260.18067197089489034Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG101230.16893980799639524Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCCG101080.16868947735133488Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCCG88650.14794541123066718Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCG83070.13863311123442212Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTAGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG69520.11601990963063713Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAATAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG68950.11506865317940781Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG66110.11032905963293183Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC65190.10879369834322834Illumina Single End PCR Primer 1 (98% over 50bp)
GATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG64460.10757542253726797Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGAGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG62240.1038705289903748Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGTGAAAGAGTGTAGATCTCGGTGGTCGCCG60420.1008331838303092Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAATAGTGTAGATCTCGGTGGTCGCC60170.10041596608854195Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)