FastQCFastQC Report
Wed 20 Sep 2023
EGAF00001348523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33230124
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT21348586.424465945417477TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA14616814.398662490696694TruSeq Adapter, Index 16 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT681730.20515421489248734TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTAT513070.1543990627299495TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTA373540.11241005299889943TruSeq Adapter, Index 16 (97% over 40bp)

[WARN]Adapter Content

Adapter graph