FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001348525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001348525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49326444
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG991736920.105582717456784TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC919568718.64250948233771TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC783470.15883366739349788TruSeq Adapter, Index 2 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC762400.1545621249324196TruSeq Adapter, Index 2 (98% over 50bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG552790.11206767712669495TruSeq Adapter, Index 2 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)