FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001386254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001386254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16521537
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC600492136.346019138534146TruSeq Adapter, Index 9 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTCGTATGCCG624720.37812462605628033TruSeq Adapter, Index 9 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGTATGCCGT525290.3179425739869117TruSeq Adapter, Index 9 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGTCTCGTATGCCG442680.2679411727855586TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTTCGTATGCC377870.22871358760386518TruSeq Adapter, Index 9 (98% over 51bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC326870.19784478889585153TruSeq Adapter, Index 9 (98% over 51bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC318480.1927665688731018TruSeq Adapter, Index 9 (100% over 46bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGATCAGATCTCGTATGCC210220.12723997773330653TruSeq Adapter, Index 9 (98% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)