FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001386271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001386271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9303152
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG211068122.687805165389108TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGC241290.2593637081281699TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACCTCTGAACTCCAGTCACATGTCAGAATCTCGTATG177650.19095678539918515TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATTCGTATGC168010.18059470596632196TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGTATGCC113740.12225963845371977TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAAATCTCGTATGC95360.10250289364292878TruSeq Adapter, Index 15 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)